Avian Influenza A Hemagglutinin antibody [B519M] (ab42904)

Product Name 

Avian Influenza A Hemagglutinin antibody [B519M]

Product type 

Primary antibodies

Description 

Mouse monoclonal [B519M] to Avian Influenza A Hemagglutinin 5

Immunogen 

Purified Avian Influenza Virus Type A (H5N1).

Species Reactivity 

Reacts with Influenza A Virus (Avian) Hemagglutinin.
Not yet tested in other species.

Specificity 

ab42904 reacts with Hemagglutinin H5 in Hemagglutinin Inhibition (HI) test. Reacts with H5N1, H5N2 and H5N9 strains. Does not cross react with other hemagglutinin types (H1, H2, H3, H4, H6, H7, H8, H9, H10, H11, H12, H13, H14 and H15).

Tested applications 
(see key)

Dot, ELISA

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
Hemagglutinin inhibition test. Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Microbiology >> Organism >> Virus >> RNA Virus >> single stranded RNA negative strand virus >> Influenza

Relevance 

Influenza A Hemagglutinin 3 binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
The extent of infection into host organism is determined by HA. Influenza viruses bud from the apical surface of polarized epithelial cells (e.g. bronchial epithelial cells) into lumen of lungs and are therefore usually pneumotropic. The reason is that HA is cleaved by tryptase clara which is restricted to lungs. However, HAs of H5 and H7 pantropic avian viruses subtypes can be cleaved by furin and subtilisin-type enzymes, allowing the virus to grow in other organs than lungs.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

B519M

Isotype 

IgG2a

Purity 

Protein G purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

>95% pure. Purity is tested by electrophoresis.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Myeloma: Hybridization of Sp 2/0 myeloma cells with spleen cells from Balb/c mice

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