Anti-Avian Influenza A Hemagglutinin antibody (ab21297)
Key features and details
- Rabbit polyclonal to Avian Influenza A Hemagglutinin
- Suitable for: WB, ELISA
- Reacts with: Influenza A
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Avian Influenza A Hemagglutinin antibody
See all Avian Influenza A Hemagglutinin primary antibodies -
Description
Rabbit polyclonal to Avian Influenza A Hemagglutinin -
Host species
Rabbit -
Specificity
Rabbit anti-Hemagglutinin (NT) protein polyclonal antibody was raised against a synthetic peptide corresponding to 15 amino acids at the amino terminus of the Hemagglutinin protein (Genbank accession no. AAT76166). Efforts were made to use relatively conserved regions of the viral sequence as the antigen. The antibody only recognizes the cleaved subunit not the full-length H5. -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Influenza A -
Immunogen
Synthetic peptide corresponding to 15 amino acids near the N terminus of the Hemagglutinin protein. (Peptide available as ab39772).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 64 kDa.
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ELISA |
Use a concentration of 1 µg/ml.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 64 kDa. |
ELISA
Use a concentration of 1 µg/ml. |
Target
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Relevance
Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. HA interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues. Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species-jumping ability. -
Cellular localization
Cell Membrane -
Database links
- GenBank: AGL44440 Influenza A
- SwissProt: P03453 Influenza A
- SwissProt: P16060 Influenza A
- SwissProt: P87506 Influenza A
- SwissProt: R4NN21 Influenza A
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Alternative names
- HA1 antibody
- HA2 antibody
- Hemagglutinin antibody
see all
Images
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H5N1 HA Antibody (ab21297 at 1 µg/ml) specifically recognizes Avian Influenza A (H5N1), but not seasonal influenza A (H1N1), recombinant Hemagglutinin protein in ELISA.
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Western blot analysis of 5 ng (A) or 25 ng (B) of recombinant Hemagglutinin (HA1) using H5N1 Hemagglutinin antibody (ab21297 at 1 µg/ml.)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab21297 has been referenced in 6 publications.
- Lin SC et al. Glycan masking of hemagglutinin for adenovirus vector and recombinant protein immunizations elicits broadly neutralizing antibodies against H5N1 avian influenza viruses. PLoS One 9:e92822 (2014). WB . PubMed: 24671139
- Lin SC et al. Heterologous prime-boost immunization regimens using adenovirus vector and virus-like particles induce broadly neutralizing antibodies against H5N1 avian influenza viruses. Biotechnol J 8:1315-22 (2013). PubMed: 23813782
- Lin SC et al. Broader Neutralizing Antibodies against H5N1 Viruses Using Prime-Boost Immunization of Hyperglycosylated Hemagglutinin DNA and Virus-Like Particles. PLoS One 7:e39075 (2012). WB . PubMed: 22720032
- Pan YS et al. Construction and characterization of insect cell-derived influenza VLP: cell binding, fusion, and EGFP incorporation. J Biomed Biotechnol 2010:506363 (2010). WB . PubMed: 21197092
- Poon LL et al. Vaccinia virus-based multivalent H5N1 avian influenza vaccines adjuvanted with IL-15 confer sterile cross-clade protection in mice. J Immunol 182:3063-71 (2009). WB . PubMed: 19234203
- Guo Y et al. Analysis of hemagglutinin-mediated entry tropism of H5N1 avian influenza. Virol J 6:39 (2009). PubMed: 19341465