Anti-Avian Influenza A Neuraminidase antibody (ab21305)
Key features and details
- Rabbit polyclonal to Avian Influenza A Neuraminidase
- Suitable for: ELISA, WB
- Reacts with: Influenza A
- Isotype: IgG
Overview
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Product name
Anti-Avian Influenza A Neuraminidase antibody
See all Avian Influenza A Neuraminidase primary antibodies -
Description
Rabbit polyclonal to Avian Influenza A Neuraminidase -
Host species
Rabbit -
Specificity
ab21305 can be used for the detection of the Neuraminidase protein from the H5N1 strain of avian influenza A in ELISA and WB. It will detect 10 ng of free peptide at 1 μg/mL.
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Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Influenza A -
Immunogen
Synthetic peptide corresponding to 16 amino acids in the middle of the Neuraminidase protein. Efforts were made to use relatively conserved regions as the antigen.
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Positive control
- WB: Avian Influenza Neuraminidase recombinant protein. ELISA: Avian Influenza Neuraminidase recombinant protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use a concentration of 1 µg/ml.
Detects 10 ng of free peptide. |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.
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Notes |
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ELISA
Use a concentration of 1 µg/ml. Detects 10 ng of free peptide. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. |
Target
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Relevance
Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication. -
Cellular localization
Cell Membrane; Virion membrane. Apical cell membrane; Single-pass type II membrane protein (By similarity). -
Database links
- Entrez Gene: 3654619 Influenza A
- Entrez Gene: 956530 Influenza A
- SwissProt: P03468 Influenza A
- SwissProt: Q710U6 Influenza A
- SwissProt: Q9Q0U7 Influenza A
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Alternative names
- NA antibody
- Neuraminidase antibody
Images
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All lanes : Anti-Avian Influenza A Neuraminidase antibody (ab21305) at 1 µg/ml
Lane 1 : 50 ng of Avian Influenza Neuraminidase recombinant protein
Lane 2 : 100 ng of Avian Influenza Neuraminidase recombinant protein
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 51 kDa1h incubation at RT in 5% NFDM/TBST.
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Validation with Avian Influenza NA Protein Coating Antigen: Avian Influenza Neuraminidase recombinant protein, 2 μg/mL, incubated at 4˚C overnight. Detection Antibodies: ab21305, dilution: 1-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1/10000 dilution, incubated at RT for 1 hr.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab21305 has been referenced in 3 publications.
- Wu CY et al. A VLP vaccine induces broad-spectrum cross-protective antibody immunity against H5N1 and H1N1 subtypes of influenza A virus. PLoS One 7:e42363 (2012). PubMed: 22879951
- Wu CY et al. Mammalian expression of virus-like particles for advanced mimicry of authentic influenza virus. PLoS One 5:e9784 (2010). WB, ICC/IF . PubMed: 20339535
- Su CY et al. In vitro evaluation of neuraminidase inhibitors using the neuraminidase-dependent release assay of hemagglutinin-pseudotyped viruses. Antiviral Res 79:199-205 (2008). PubMed: 18453004