Anti-Avian Influenza Matrix Protein I antibody (ab25918)
Key features and details
- Rabbit polyclonal to Avian Influenza Matrix Protein I
- Suitable for: WB, ICC/IF
- Reacts with: Influenza A
- Isotype: IgG
Overview
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Product name
Anti-Avian Influenza Matrix Protein I antibody
See all Avian Influenza Matrix Protein I primary antibodies -
Description
Rabbit polyclonal to Avian Influenza Matrix Protein I -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Influenza A -
Immunogen
Synthetic peptide:
SGPLKAEIAQRLEDVFAGKN
, corresponding to amino acids 9-28 of Chicken Avian Influenza Matrix Protein 1. -
Positive control
- Recombinant fusion protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituent: 0.05% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
This antibody was purified by chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 27 kDa.
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ICC/IF |
1/10.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 27 kDa. |
ICC/IF
1/10. |
Target
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Relevance
An H6N1 influenza virus was isolated from a green-winged teal during the H5N1 outbreak in Hong Kong Special Administrative Region (SAR) in 1997. This virus possesses similar genes encoding internal proteins as in the human H5N1 and H9N2 influenza viruses. In 1999, influenza viruses from quail infected two humans in Hong Kong, suggesting the potential for avian influenza viruses to cross the species barrier and infect humans without prior reassortment in an intermediate host, such as the pig. The common features shared by H5N1 and H9N2 influenza viruses isolated from humans are the genes encoding the proteins of the replicating complex, the matrix protein (M) gene, the nonstructural protein (NS) gene, N1 neuraminidase (NA), This virus essentially represents the reemergence of the H5N1 influenza viruses with a different hemagglutinin (HA). -
Database links
- SwissProt: Q809Z8 Influenza A
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Alternative names
- M antibody
- M1 antibody
- M1 matrix protein 1 antibody
- Matrix protein 1 antibody
Images
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All lanes : Anti-Avian Influenza Matrix Protein I antibody (ab25918) at 0.5 µg/ml (Western blot analysis of Avian Flu Matrix Protein 1.)
Lane 1 : Recombinant fusion protein containing amino acids 9-28 (A). at 0.1 µg
Lane 2 : Fusion partner without these amino acids (B).
Predicted band size: 27 kDa -
Immunofluoroscence staining of influenza infected MDCK cells using ab25918 at 1/10 dilution. Image courtesy of Catherine Thompson, The University of Reading.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab25918 has been referenced in 3 publications.
- Mizuno K et al. Nicotinic acetylcholine receptors regulate type 1 inositol 1,4,5-trisphosphate receptor expression via calmodulin kinase IV activation. J Neurosci Res 93:660-5 (2015). Flow Cyt ; Human . PubMed: 25430056
- Lin SC et al. Broader Neutralizing Antibodies against H5N1 Viruses Using Prime-Boost Immunization of Hyperglycosylated Hemagglutinin DNA and Virus-Like Particles. PLoS One 7:e39075 (2012). WB . PubMed: 22720032
- Pan YS et al. Construction and characterization of insect cell-derived influenza VLP: cell binding, fusion, and EGFP incorporation. J Biomed Biotechnol 2010:506363 (2010). WB . PubMed: 21197092