Avian Influenza Nucleoprotein antibody (ab22285)

Product Name 

Avian Influenza Nucleoprotein antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Avian Influenza Nucleoprotein

Immunogen 

Synthetic peptide, corresponding to amino acids 428-441 of Avian Influenza Nucleoprotien.

Tested applications 
(see key)

ELISA, ICC/IF

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 0.1 µg/ml.
ICC/IF: 1/10.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Microbiology >> Organism >> Virus >> RNA Virus >> single stranded RNA negative strand virus >> Influenza

Relevance 

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein G purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 0.2% Gelatin, PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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References for Avian Influenza Nucleoprotein antibody (ab22285)

  This product has been used in:

Yamamoto Y et al. Detecting avian influenza virus (H5N1) in domestic duck feathers. Emerg Infect Dis 14:1671-2 (2008). PubMed: 18826847

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