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Immunocytochemistry/ Immunofluorescence - Avian Influenza Nucleoprotien antibody (ab22285)
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Product Name
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Avian Influenza Nucleoprotein antibody
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See all Avian Influenza Nucleoprotein antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Avian Influenza Nucleoprotein
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Immunogen
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Synthetic peptide, corresponding to amino acids 428-441 of Avian Influenza Nucleoprotien.
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Tested applications
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ELISA, ICC/IF
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at a concentration of 0.1 µg/ml.
ICC/IF: 1/10.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Microbiology >> Organism >> Virus >> RNA Virus >> single stranded RNA negative strand virus >> Influenza
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Relevance
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Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus
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Avian Influenza Nucleoprotein antibody (ab22285) |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 0.2% Gelatin, PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Avian Influenza Nucleoprotein antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Avian Influenza Nucleoprotein antibody images, references, products related to ab22285 and other tools.
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Avian Influenza Nucleoprotein antibody images:
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 Immunocytochemistry/ Immunofluorescence - Avian Influenza Nucleoprotien antibody (ab22285)
Immunofluoroscence staining of influenza−infected MDCK cells using ab22285 at 1:10 dilution. Image Courtesy of Catherine Thompson, The University of Reading
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References for Avian Influenza Nucleoprotein antibody (ab22285)
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This product has been used in:
Yamamoto Y et al. Detecting avian influenza virus (H5N1) in domestic duck feathers. Emerg Infect Dis 14:1671-2 (2008). PubMed: 18826847
If you publish research using ab22285 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Avian Influenza Nucleoprotein
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Avian Influenza Nucleoprotein antibody - more information
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