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Products:Microbiology >> Organism >> Virus >> RNA Virus >> single stranded RNA negative strand virus >> Influenza
Overview
Product name
Anti-Avian Influenza Nucleoprotein antibody
See all Avian Influenza Nucleoprotein products (2) ...
Description
Rabbit polyclonal to Avian Influenza Nucleoprotein
Tested applications
ELISA, ICC/IFmore details
Immunogen
Synthetic peptide, corresponding to amino acids 428-441 of Avian Influenza Nucleoprotein
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Microbiology >> Organism >> Virus >> RNA Virus >> single stranded RNA negative strand virus >> Influenza
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Immunocytochemistry/ Immunofluorescence - Avian Influenza Nucleoprotien antibody (ab22285)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab22285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use a concentration of 0.1 µg/ml
ICC/IF: 1/10
Target
Relevance
Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus
Cellular localization
Nuclear
Alternative names
- NP antibody
- Nucleocapsid protein antibody
- Nucleoprotein antibody
- Protein N antibody
Anti-Avian Influenza Nucleoprotein antibody images:
Immunocytochemistry/ Immunofluorescence - Avian Influenza Nucleoprotien antibody (ab22285)
Immunofluoroscence staining of influenza−infected MDCK cells using ab22285 at 1:10 dilution.
Image Courtesy of Catherine Thompson, The University of Reading
References for Anti-Avian Influenza Nucleoprotein antibody (ab22285)
This product has been referenced in:
- Yamamoto Yet al. Detecting avian influenza virus (H5N1) in domestic duck feathers. Emerg Infect Dis 14:1671-2 (2008).Read more (PubMed: 18826847) »
See 1 publication for this product
Publishing research using ab22285? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"