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Products:Microbiology >> Organism >> Virus >> RNA Virus >> single stranded RNA negative strand virus >> Influenza
Overview
Product name
Anti-Avian Influenza Nucleoprotein antibody
See all Avian Influenza Nucleoprotein products (2) ...
Description
Rabbit polyclonal to Avian Influenza Nucleoprotein
Tested applications
Immunogen
Synthetic peptide: kls dyegrliqns itiermv, corresponding to amino acids 58-77 of Avian Influenza Nucleoprotein.
kls dyegrliqns itiermv
Positive control
Recombinant fusion protein of avian flu nucleoprotein.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Microbiology >> Organism >> Virus >> RNA Virus >> single stranded RNA negative strand virus >> Influenza
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Western blot - Avian Influenza Nucleoprotein antibody (ab25921)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25921 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 0.5-2.0 µg/ml.
Please note from the image legend that a recombinant fusion protein was used to generate the western blot image.
The molecular weight shown is the molecular weight of the fusion protein (e.g nucleoprotein amino acids 58-77 + fusion protein) not the Avian Influenza Nucleoprotein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus
Cellular localization
Nuclear
Alternative names
- NP antibody
- Nucleocapsid protein antibody
- Nucleoprotein antibody
- Protein N antibody
Anti-Avian Influenza Nucleoprotein antibody images:
Western blot - Avian Influenza Nucleoprotein antibody (ab25921)
All lanes : Anti-Avian Influenza Nucleoprotein antibody (ab25921) at 0.5 µg/ml
Lane 1 : Recombinant fusion protein containing amino acids 58-77 of Avian flu nucleoprotein at 0.1 µg
Lane 2 : Fusion partner without these amino acids
Observed band size : 31 kDa (why is the actual band size different from the predicted?)
Additional bands at : 56 kDa,59 kDa,66 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Avian Influenza Nucleoprotein antibody (ab25921)
ab25921 has not yet been referenced specifically in any publications.
Publishing research using ab25921? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"