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Overview

  • Product nameAvidin protein (Active)See all Avidin proteins and peptides ...
  • Protein descriptionFull length (Chicken) Avidin; a glycosylated tetramer, each subunit with a binding site for biotin. Produced from Hen's egg white, 68kDa. SwissProt P02701.
  • Expression hostNative

    • Properties

  • Purification notesab73656 is purified by affinity chromatographic techniques.
  • Biological activityActivity: Greater than 11 U/mg, 1 unit binds 1µg biotin.
  • FormLyophilised:Reconstitute in sterile 18MOhm/cm H2O at not less than 100µg/ml, or more than 10mg/ml solution.
  • Storage instructionsStore at +4°C.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
  • Concentration information loading...
  • Additional notesFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab73656 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent dilution. Ab73656 can be used as a WB positive control in conjunction with ab6675.
    ELISA ELISA: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        AVDAVID_CHICKAvidinLOC396260
  • FunctionThe biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
  • Tissue specificitySynthesized in hen oviduct and concentrated in egg white (where it represent 0.05% of the total protein).
  • Sequence similaritiesBelongs to the avidin/streptavidin family.
    Contains 1 avidin-like domain.
  • Post-translational
    modifications    N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)][Man(alpha1-?)Man(alpha1-6)] Man(beta1-4)GlcNAc(beta1-4)GlcNAc.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P02701 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Avidin protein (Active) images

    • Western blot - Anti-Avidin antibody (ab6675)
      Western blot - Anti-Avidin antibody (ab6675)
      Anti-Avidin antibody (ab6675) at 1/1000 dilution + Avidin protein (Active) (ab73656) at 0.001 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 30 seconds

    References for Avidin protein (Active) (ab73656)

    ab73656 has not yet been referenced specifically in any publications.

    Publishing research using ab73656 ? Please let us know so that we can cite the reference in this datasheet

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