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Axl 293T Transfected Lysate - (positive control) (ab94145)

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Overview

  • Product nameAxl 293T Transfected Lysate - (positive control)
  • General notesab94145 is a 293T cell transfected lysate in which Human Axl has been transiently over-expressed using a pCMV-Axl plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundFunction: May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor. Tissue specificity: Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily. Contains 2 fibronectin type-III domains. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain.

    • Applications

    Our Abpromise guarantee covers the use of ab94145 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Axl 293T Transfected Lysate - (positive control) images

    • SDS-PAGE - Axl 293T Transfected Lysate - (positive control) (ab94145)
      SDS-PAGE - Axl 293T Transfected Lysate - (positive control) (ab94145)
      ab94145 at 15µg/lane on an SDS-PAGE gel.
    • Western blot - Axl 293T Transfected Lysate - (positive control) (ab94145)
      Western blot - Axl 293T Transfected Lysate - (positive control) (ab94145)
      All lanes : Anti-Axl antibody (ab54803) at 1/500 dilution

      Lane 1 : Axl 293T Transfected Lysate - (positive control) (ab94145)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for Axl 293T Transfected Lysate - (positive control) (ab94145)

    ab94145 has not yet been referenced specifically in any publications.

    Publishing research using ab94145 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"