Axl Mouse ELISA Kit (ab100669)
- Product nameAxl Mouse ELISA KitSee all Axl kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 1 pg/ml
- Range1 pg/ml - 250 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 93.26 81% - 105% Serum 95.29 82% - 104% Plasma 97.56 84% - 106%
- Species reactivityReacts with: Mouse
- Product overview
ab100669 Axl Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Axl in serum, plasma and cell culture supernates. This assay employs an antibody specific for mouse Axl coated on a 96-well plate. Standards and samples are pipetted into the wells and Axl present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Axl antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Axl bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent A animal serum . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Axl Microplate (12 trips x 8 wells) coated with antimouse Axl 1 unit Axl Standard Mouse(recombinant) 2 vials Detection Antibody Axl : biotinylated anti-mouse Axl 2 vials HRP-Streptavidin Concentrate 80x concentrated HRP conjugated streptavidin Item G 1 x 200µl Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Adhesion related kinaseAI323647 Ark
- AXL oncogeneAXL receptor tyrosine kinaseAXL transforming gene AXL transforming sequence/gene JTK11Oncogene AXLTyro7 Tyrosine protein kinase receptor UFO UFO
- SwissProt: Q00993 Mouse
Our Abpromise guarantee covers the use of ab100669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Axl Mouse ELISA Kit (ab100669)
ab100669 has not yet been referenced specifically in any publications.