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Overview

  • Product nameAxl Mouse ELISA KitSee all Axl kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 1 pg/ml
  • Range
    1 pg/ml - 250 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 93.26 81% - 105%
    Serum 95.29 82% - 104%
    Plasma 97.56 84% - 106%

  • Species reactivity
    Reacts with: Mouse
  • Product overview

    ab100669 Axl Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Axl in serum, plasma and cell culture supernates. This assay employs an antibody specific for mouse Axl coated on a 96-well plate. Standards and samples are pipetted into the wells and Axl present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Axl antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Axl bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsELISA more details

Properties

  • RelevanceThe protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors such as vitamin K dependent protein growth arrest specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia associated oncogene and also associated with colon cancer and melanoma. It is in close vicinity to the bcl3 oncogene which is at 19q13.1-q13.2. The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants (AXL1 and AXL2).
    • Alternative names
        Adhesion related kinaseAI323647 Ark
        AXL oncogeneAXL receptor tyrosine kinaseAXL transforming gene AXL transforming sequence/gene JTK11Oncogene AXLTyro7 Tyrosine protein kinase receptor UFO UFO
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100669 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Axl Mouse ELISA Kit images

      • ELISA - Axl Mouse ELISA Kit (ab100669)
        ELISA - Axl Mouse ELISA Kit (ab100669)

      Protocols

      References for Axl Mouse ELISA Kit (ab100669)

      ab100669 has not yet been referenced specifically in any publications.

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