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Western blot - Axl antibody (ab37861)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Axl antibody (ab37861)

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Product Name
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Axl antibody
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See all Axl antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Axl
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Immunogen
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Synthetic peptide: RGTQAEESPFVGNPGNI conjugated to KLH, corresponding to N terminal amino acids 21-37 of Axl BLAST 'RGTQAEESPFVGNPGNI' with or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: 1/1000. WB: 1/100 - 1/500. Detects a band of approximately 97 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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SKBR3 cell lysate.
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Cellular localization
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Cell Membrane; single pass type I membrane protein.
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Research areas
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Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers Cancer >> Signal transduction >> Other Cancer >> Cell cycle >> Kinases/phosphatases >> Other Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases Tags & Cell Markers >> Cell Type Markers >> Tumor Associated Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
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Relevance
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The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors such as vitamin K dependent protein growth arrest specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia associated oncogene and also associated with colon cancer and melanoma. It is in close vicinity to the bcl3 oncogene which is at 19q13.1-q13.2. The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants (AXL1 and AXL2).
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.09% Sodium Azide Constituents: PBS Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Axl antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Axl antibody images, references, products related to ab37861 and other tools.
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Axl antibody images:
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Western blot - Axl antibody (ab37861)
Axl antibody (ab37861) at 1/1000 dilution + SKBR3 cell lysate
Secondary HRP-anti-rabbit developed using the ECL technique
Observed band size : 97 kDa (why is the actual band size different from the predicted?) Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Axl antibody (ab37861)
ab37861 (1/50) staining human breast carcinoma tissue by immunohistochemistry, formalin-fixed, paraffin-embedded tissue.
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Search PubMed (MEDLINE) for references to Axl
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Axl antibody - more information
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Customer reviews (feedback) regarding Axl antibody |
Customer FAQs regarding Axl antibody |
Protocols for Axl antibody |
Price and availability of products related to Axl antibody |
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Axl antibody - related products:
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Axl antibody - other tools:
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Contact Abcam with a Technical Enquiry about Axl antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"