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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
Overview
Product name
Anti-Axl antibody
See all Axl products (8) ...
Description
Rabbit polyclonal to Axl
Tested applications
IHC-P, Flow Cyt, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: RGTQAEESPFVGNPGNI conjugated to KLH, corresponding to N terminal amino acids 21-37 of Axl
RGTQAEESPF VGNPGNI
Positive control
SKBR3 cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Signal transduction >> Other
Cancer >> Cell cycle >> Kinases/phosphatases >> Other
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
Flow Cyt
Flow Cyt: 1/10 - 1/50.Flow Cyt: 1/10 - 1/50.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/100 - 1/500.Detects a band of approximately 97 kDa.
Target
Function
May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor.
Tissue specificity
Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionP30530
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Adhesion related kinase antibody
- AI323647 antibody
- Ark antibody
see all
Database links
- Entrez Gene: 558 Human
- Omim: 109135 Human
- SwissProt: P30530 Human
- Unigene: 590970 Human
Anti-Axl antibody images:
Western blot - Axl antibody (ab37861)
Anti-Axl antibody (ab37861) at 1/1000 dilution + SKBR3 cell lysate
Secondary
HRP-anti-rabbit
developed using the ECL technique
Observed band size : 97 kDa (why is the actual band size different from the predicted?)
Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Axl antibody (ab37861)
ab37861 (1/50) staining human breast carcinoma tissue by immunohistochemistry, formalin-fixed, paraffin-embedded tissue.
References for Anti-Axl antibody (ab37861)
ab37861 has not yet been referenced specifically in any publications.
Publishing research using ab37861? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"