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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Axl antibody (ab54803)
Predicted band size : 97 kDa
Axl antibody (ab54803) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Western blot - Axl antibody (ab54803)
All lanes : Anti-Axl antibody (ab54803) at 1/2000 dilution (in PBS + 3% BSA + 0.02% sodium azide for 16 hours at 4°C)
Lane 1 : Whole cell lysate of MCF7 infected with human Axl expressing lentivirus (high expression)
Lane 2 : Whole cell lysate of MCF7 infected with human Axl expressing lentivirus (low expression)
Lane 3 : Whole cell lysate of non-infected MCF7
Lysates/proteins at 10 µg per lane.
Secondary
An HRP-conjugated Donkey anti-mouse polyclonal at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 97 kDa
Observed band size : ~100 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 minutes
Blocking Step: 5% Milk for 1 hour at 22°C
This image is courtesy of an Abreview submitted by Nir Drayman
Axl antibody for Western blot in African Green Monkey (54803)
Axl antibody for Western blot in Human (54803)
Axl antibody for Immunoprecipitation in African Green Monkey (54803)
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