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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
Overview
Product name
Anti-Axl antibody
See all Axl products (8) ...
Description
Mouse monoclonal to Axl
Tested applications
Cross reactivity
Reacts with
Human, African Green Monkey
Immunogen
Recombinant fragment, corresponding to amino acids 30-140 of Human Axl
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Signal transduction >> Other
Cancer >> Cell cycle >> Kinases/phosphatases >> Other
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor.
Tissue specificity
Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionP30530
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Adhesion related kinase antibody
- AI323647 antibody
- Ark antibody
see all
Database links
- Entrez Gene: 558 Human
- Omim: 109135 Human
- SwissProt: P30530 Human
- Unigene: 590970 Human
Anti-Axl antibody images:
Western blot - Axl antibody (ab54803)
Predicted band size : 97 kDa
Axl antibody (ab54803) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Western blot - Axl antibody (ab54803)
All lanes : Anti-Axl antibody (ab54803) at 1/2000 dilution (in PBS + 3% BSA + 0.02% sodium azide for 16 hours at 4°C)
Lane 1 : Whole cell lysate of MCF7 infected with human Axl expressing lentivirus (high expression)
Lane 2 : Whole cell lysate of MCF7 infected with human Axl expressing lentivirus (low expression)
Lane 3 : Whole cell lysate of non-infected MCF7
Lysates/proteins at 10 µg per lane.
Secondary
An HRP-conjugated Donkey anti-mouse polyclonal at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 97 kDa
Observed band size : ~100 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 minutes
Blocking Step: 5% Milk for 1 hour at 22°C
This image is courtesy of an Abreview submitted by Nir Drayman
References for Anti-Axl antibody (ab54803)
ab54803 has not yet been referenced specifically in any publications.
Publishing research using ab54803? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"