Overview
- Product nameAnti-Azurocidin antibodySee all Azurocidin primary antibodies ...
- DescriptionRabbit polyclonal to Azurocidin
- Tested applicationsWB, ICC/IF more details
- Species reactivityReacts with: Human
- Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Azurocidin.
(Peptide available as ab88967.)
- Positive controlThis antibody gave a positive signal in Human Thymus Tissue Lysate.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab88819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).Can be blocked with Azurocidin peptide (ab88967). |
| ICC/IF | ICC/IF: Use a concentration of 1 µg/ml. |
Target
- FunctionThis is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.
- Sequence similaritiesBelongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. - Cellular localizationCytoplasmic granule. Cytoplasmic granules of neutrophils.
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Database links
- Entrez Gene: 566 Human
- Omim: 162815 Human
- SwissProt: P20160 Human
- Unigene: 72885 Human
Target information above from: UniProt accession
P20160
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Azu 1 antibodyAzu1 antibodyAzurocidin antibody
- CAP37 antibodyCAP7_HUMAN antibodycationic antimicrobial protein 37 antibodyCationic antimicrobial protein CAP37 antibodyHBP antibodyHeparin binding protein antibodyHeparin-binding protein antibodyHUMAZUR antibodyNeutrophil azurocidin antibody
see all
Anti-Azurocidin antibody images
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Western blot - Anti-Azurocidin antibody (ab88819)Anti-Azurocidin antibody (ab88819) at 1 µg/ml +Thymus (Human) Tissue Lysate - adult normal tissue (ab30146) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 27 kDa
Observed band size : 27 kDa
Exposure time : 6 minutes -
Immunocytochemistry/ Immunofluorescence - Azurocidin antibody (ab88819)ICC/IF image of ab88819 stained Hek293 cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88819, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) MCF-7 cells at 1µg/ml, and in 4% PFA fixed (10 min) Hek293 cells at 1µg/ml.
References for Anti-Azurocidin antibody (ab88819)
ab88819 has not yet been referenced specifically in any publications.
