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Azurocidin protein (Human) (ab91129)

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Overview

  • Product nameAzurocidin protein (Human)See all Azurocidin proteins and peptides ...
  • Protein descriptionFull length native Human Azurocidin purified from Human neutrophils; amino acid 27-248, 222 amino acids, MWt 37kDa (UniProt P20160).
  • Expression hostNative

    • Properties

  • Purity> 95 % by SDS-PAGE
  • FormLyophilised
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferDe-ionized H2O
  • Concentration information loading...
  • Additional notesPrepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91129 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent concentration. Ab91129 can be used as a WB positive control in conjunction with ab48132.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        Azu 1Azu1Azurocidin
        CAP37CAP7_HUMANcationic antimicrobial protein 37Cationic antimicrobial protein CAP37HBPHeparin binding proteinHeparin-binding proteinHUMAZURNeutrophil azurocidin
      see all
  • FunctionThis is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.
  • Sequence similaritiesBelongs to the peptidase S1 family. Elastase subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localizationCytoplasmic granule. Cytoplasmic granules of neutrophils.

    • Target information above from: UniProt accession P20160 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Azurocidin protein (Human) images

    • Western blot - Anti-Azurocidin antibody (ab48132)
      Western blot - Anti-Azurocidin antibody (ab48132)
      Anti-Azurocidin antibody (ab48132) at 1 µg/ml + Azurocidin protein (Human) (ab91129) at 0.1 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 2 minutes
    • SDS-PAGE - Azurocidin protein (Human) (ab91129)
      SDS-PAGE - Azurocidin protein (Human) (ab91129)
      Lane 1. LMW Standard
      Lane 2. Azurocidin - 5 µg (reduced / heated)
      Lane 3. Azurocidin - 10 µg (reduced / heated)
      Lane 4. Azurocidin - 20 µg (reduced / heated)
      4-12% Bis-Tris SDS-PAGE gel

    References for Azurocidin protein (Human) (ab91129)

    ab91129 has not yet been referenced specifically in any publications.

    Publishing research using ab91129 ? Please let us know so that we can cite the reference in this datasheet

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    Dear Sir/Madam, I have ordered Human Azurocidin protein (Ab91129) which arrived yesterday. Would you please let me know which buffer is this protein lyophilised from? Thanks  

    Thank you for contacting us. This protein is lyophilised from de-ionized water, and reconstituted to pre-lyophilisation volume with de-ionized water. I hope this information is helpful to you. Please do not hesitate to contact us if you nee...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"