Anti-B MyB (phospho S577 + S581) antibody (ab61000)

Overview

• Product nameAnti-B MyB (phospho S577 + S581) antibodySee all B MyB primary antibodies ...
• Description
  Rabbit polyclonal to B MyB (phospho S577 + S581)
• SpecificityDetects endogenous levels of B MyB only when phosphorylated at serine 577/581.
• Tested applicationsELISA, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthetic phosphopeptide derived from human B MyB around the phosphorylation site of serine 577/581 (R-R-S^P-P-I).

• Positive controlExtracts from K562 cells.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesAffinity purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Transcription factors

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cell Differentiation

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Transcription Factors

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Cell Biology
  • Cell Cycle
  • Cell differentiation

Applications

Target

• FunctionTranscription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.
• Sequence similaritiesContains 3 HTH myb-type DNA-binding domains.
• Post-translational
  modificationsPhosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity.
• Cellular localizationNucleus.

Target information above from: UniProt accession P10244 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4605 Human
  • Entrez Gene: 17865 Mouse
  • Entrez Gene: 296344 Rat
  • Omim: 601415 Human
  • SwissProt: P10244 Human
  • SwissProt: P48972 Mouse
  • Unigene: 179718 Human
  • Unigene: 4594 Mouse

  • Unigene: 20824 Rat

  see all

• Alternative names
  B-Myb antibodyBMyB antibodyMGC15600 antibody

  MYB L2 antibodyMyb related protein B antibodyMyb-like protein 2 antibodyMyb-related protein B antibodyMybB antibodyMYBB_HUMAN antibodyMYBL 2 antibodyMYBL2 antibodyv myb avian myeloblastosis viral oncogene homolog like 2 antibodyv myb myeloblastosis viral oncogene homolog (avian) like 2 antibodyv myb myeloblastosis viral oncogene homolog like 2 antibody

  see all

Anti-B MyB (phospho S577 + S581) antibody images

• 

  Western blot - B MyB (phospho S577 + S581) antibody (ab61000)
  All lanes : Anti-B MyB (phospho S577 + S581) antibody (ab61000) at 1/500 dilution
  
  Lane 1 : extracts from K562 cells
  Lane 2 : extracts from K562 cells with immunizing phosphopeptide
  
  
  Predicted band size : 79 kDa
  Observed band size : 79 kDa