Anti-B Raf antibody [1D2] (ab114014)

Overview

• Product nameAnti-B Raf antibody [1D2]See all B Raf primary antibodies ...
• Description
  Mouse monoclonal [1D2] to B Raf
• Tested applicationsWB, Flow Cyt, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Full length Human recombinant B Raf (NP_004324) produced in HEK293T cells.

• Positive controlWB: lysate from transfected HEK293T cells. IF: transfected COS7 cells. FC: HeLa or Jurkat cells.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferpH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: 48% PBS, 1% BSA, 50% Glycerol
• Concentration information loading...
• PurityIgG fraction
• Purification notesPurified from mouse ascites fluids by affinity chromatography
• Clonality Monoclonal
• Clone number1D2
• IsotypeIgG1
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Signal transducers

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Other

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Kinases

  • Cell Biology
  • Cell Cycle
  • Cell differentiation

Applications

Target

• FunctionInvolved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
• Tissue specificityBrain and testis.
• Involvement in diseaseNote=Defects in BRAF are found in a wide range of cancers.
  Defects in BRAF may be a cause of colorectal cancer (CRC) [MIM:114500].
  Defects in BRAF are involved in lung cancer (LNCR) [MIM:211980].
  Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
  Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
  Defects in BRAF are the cause of Noonan syndrome type 7 (NS7) [MIM:613706]. Noonan syndrome is a disorder characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits.
  Defects in BRAF are the cause of LEOPARD syndrome type 3 (LEOPARD3) [MIM:613707]. LEOPARD3 is a disorder characterized by lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and sensorineural deafness.
  Note=A chromosomal aberration involving BRAF is found in pilocytic astrocytomas. A tandem duplication of 2 Mb at 7q34 leads to the expression of a KIAA1549-BRAF fusion protein with a constitutive kinase activity and inducing cell transformation.
• Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
  Contains 1 phorbol-ester/DAG-type zinc finger.
  Contains 1 protein kinase domain.
  Contains 1 RBD (Ras-binding) domain.
• Cellular localizationNucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.

Target information above from: UniProt accession P15056 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 673 Human
  • Omim: 164757 Human
  • SwissProt: P15056 Human
  • Unigene: 550061 Human

• Alternative names
  FLJ95109 antibody94 kDa B raf protein antibodyB raf 1 antibody

  B Raf proto oncogene serine threonine protein kinase antibodyBRAF 1 antibodyBRAF antibodyBRAF_HUMAN antibodyBRAF1 antibodycRmil antibodyMGC126806 antibodyMGC138284 antibodyMurine sarcoma viral (v-raf) oncogene homolog B1 antibodyMurine sarcoma viral v raf oncogene homolog B1 antibodyNS7 antibodyp94 antibodyProto-oncogene B-Raf antibodyProto-oncogene c-Rmil antibodyRAFB 1 antibodyRAFB1 antibodySerine/threonine-protein kinase B-raf antibodyv raf murine sarcoma viral oncogene homolog B1 antibodyv-Raf murine sarcoma viral oncogene homolog B1 antibody

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Anti-B Raf antibody [1D2] images

• 

  Flow Cytometry - Anti-B Raf antibody [1D2] (ab114014)
  Flow cytometric analysis of Hela cells, using 1/100 ab114014 (Red) compared to a nonspecific negative control antibody (Blue).
• 

  Flow Cytometry - Anti-B Raf antibody [1D2] (ab114014)
  Flow cytometric analysis of Jurkat cells, using 1/100 ab114014 (Red) compared to a nonspecific negative control antibody (Blue).
• 

  Immunocytochemistry/ Immunofluorescence - Anti-B Raf antibody [1D2] (ab114014)
  Immunofluorescent staining of transiently transfected COS7 cells using 1/100 ab114014.
• 

  Western blot - Anti-B Raf antibody [1D2] (ab114014)
  All lanes : Anti-B Raf antibody [1D2] (ab114014) at 1/2000 dilution
  
  Lane 1 : mock-transfected HEK293T cell lysate
  Lane 2 : B Raf-transfected HEK293T cell lysate
  
  Lysates/proteins at 5 µg per lane.
  
  
  Predicted band size : 64 kDa