Overview

  • Product nameAnti-B Raf antibody [4B2]
    See all B Raf primary antibodies
  • Description
    Mouse monoclonal [4B2] to B Raf
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    Protein expressed in 293T cells, transfected with Human B Raf expression vector (NM_004333).

  • Positive control
    • HEK293T cells transfected with pCMV6-ENTRY B Raf cDNA, HeLa, HT29, COS7 and Jurkat cells
  • General notesDilute in PBS (pH7.3) before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab117860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 84 kDa.

Target

  • FunctionInvolved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
  • Tissue specificityBrain and testis.
  • Involvement in diseaseNote=Defects in BRAF are found in a wide range of cancers.
    Defects in BRAF may be a cause of colorectal cancer (CRC) [MIM:114500].
    Defects in BRAF are involved in lung cancer (LNCR) [MIM:211980].
    Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
    Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
    Defects in BRAF are the cause of Noonan syndrome type 7 (NS7) [MIM:613706]. Noonan syndrome is a disorder characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits.
    Defects in BRAF are the cause of LEOPARD syndrome type 3 (LEOPARD3) [MIM:613707]. LEOPARD3 is a disorder characterized by lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and sensorineural deafness.
    Note=A chromosomal aberration involving BRAF is found in pilocytic astrocytomas. A tandem duplication of 2 Mb at 7q34 leads to the expression of a KIAA1549-BRAF fusion protein with a constitutive kinase activity and inducing cell transformation.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 RBD (Ras-binding) domain.
  • Cellular localizationNucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ95109 antibody
    • 94 kDa B raf protein antibody
    • B raf 1 antibody
    • B Raf proto oncogene serine threonine protein kinase antibody
    • B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody
    • BRAF 1 antibody
    • BRAF antibody
    • BRAF_HUMAN antibody
    • BRAF1 antibody
    • cRmil antibody
    • FLJ95109 antibody
    • MGC126806 antibody
    • MGC138284 antibody
    • Murine sarcoma viral (v-raf) oncogene homolog B1 antibody
    • Murine sarcoma viral v raf oncogene homolog B1 antibody
    • NS7 antibody
    • Oncogen BRAF antibody
    • oncogene BRAF1 antibody
    • p94 antibody
    • Proto-oncogene B-Raf antibody
    • Proto-oncogene c-Rmil antibody
    • RAFB 1 antibody
    • RAFB1 antibody
    • RMIL antibody
    • Serine/threonine-protein kinase B-raf antibody
    • v raf murine sarcoma viral oncogene homolog B antibody
    • v raf murine sarcoma viral oncogene homolog B1 antibody
    • v-Raf murine sarcoma viral oncogene homolog B1 antibody
    see all

Anti-B Raf antibody [4B2] images

  • All lanes : Anti-B Raf antibody [4B2] (ab117860) at 1/2000 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY B Raf cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 84 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC211013)
  • All lanes : Anti-B Raf antibody [4B2] (ab117860) at 1/2000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HT29 cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : COS7 cell lysate
    Lane 6 : Jurkat cell lysate
    Lane 7 : MDCK cell lysate
    Lane 8 : PC12 cell lysate
    Lane 9 : MCF7 cell lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 84 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC211013)

References for Anti-B Raf antibody [4B2] (ab117860)

ab117860 has not yet been referenced specifically in any publications.

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