Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab33899? Please let us know so that we can cite the reference in this datasheet
ab33899 has been referenced in 3 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-B Raf antibody [EP152Y] (ab33899) at 1/5000 dilution + HeLa cell lysate
Predicted band size : 85 kDa
Observed band size : 87 kDa (why is the actual band size different from the predicted?)
This image shows paraffin embedded human prostate cancer tissue sample stained with ab33899 at 1/250 dilution.
All lanes : Anti-B Raf antibody [EP152Y] (ab33899) at 1/1000 dilution
Lane 1 : Lysate prepared from mouse brain
Lane 2 : Lysate prepared from mouse heart
Lane 3 : Lysate prepared from mouse kidney
Lane 4 : Lysate prepared from mouse spleen
Lane 5 : Lysate prepared from rat brain
Lane 6 : Lysate prepared from rat heart
Lane 7 : Lysate prepared from rat kidney
Lane 8 : Lysate prepared from rat spleen
Predicted band size : 85 kDa
Exposure time : 3 minutes
ab33899 staining B Raf cells from human prostate tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formaldehyde fixed and permeabilized in PBS-Tween 20 prior to blocking in 70% serum for 10 minutes at 25ºC. The primary antibody was diluted 1/250 and incubated with the sample for 1 hour at 25ºC. A biotin conjugated goat polyclonal to mouse Ig was used as the secondary.
This image is courtesy of an Abreview submitted by Sedar Balci
5
Call 01223 696 000 or contact us