Anti-B Raf antibody [EP152Y] (ab33899)

Thank you what is the status of our order?

ANSWER:

Unfortunately shipment of this order is being held up by our accounts team.

Our accounting department can be contacted by email at mailto:us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to theorder reference numberin any correspondence with our accounting department.

I hope this is helpful. Please contact me again with any technical questions you may have.

I wish to determine if the above product is capable of differentiating between the wild type BRAF sequence and that of the pathogenic variant related sequence BRAF V600E. Do you supply any antibodies specific to BRAF containing the V600E variant,

ANSWER:

Thank you for your enquiry.

I have been in touch with the lab and I can tell you that this antibody has NOT been tested for its capacity to differentiate between wild type and variant (V600E) BRAF. I could not find literature relating to the variant sequence of BRAF. However, I can tell you that the immunogen sequence used to raise this antibody was CGEHNPPSIYLEAYEEYT, corresponding to N terminal amino acids 70-86.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

ab33899 = B raf Ab [EP152Y]

Reacts with Rat and Human. Does not react with Mouse. Not yet tested in other species. BLAST 'CGEHNPPSIYLEAYEEYT'

Sequence is an exact match between human and mouse, so why would this Ab not react in mouse?

Surrounding sequences of immunogen might not be homologous.

Contact supplier - tested which applications in mouse, want protocols.

ANSWER:

Thank you for your enquiry and patience.

Ab33899 (B-raf antibody) was tested by Western Blotting on various mouse tissues (brain, heart, kidney, muscle) and was found to not cross-react with these tissues.

This antibody should work fine in your system, human transgene in a mouse model.

Thank you for your email. I plan to order your Braf antibody through our US purchasing office. I would like to know where is the location of immunogen. I am looking for antibody targeting c-terminal portion of Braf protein corresponding to exon 17-18.

ANSWER:

A synthetic peptide corresponding to residues near N-terminus of human B-Raf (aa-70-86) was used as an immunogen for anti-B-Raf Rabbit Monoclonal antibody ab33899. The exact peptide sequence is following: CGEHNPPSIYLEAYEEYT.

I hope this information helps you,