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Western blot - B Raf antibody [EP152Y] (ab33899)
Western blot - B Raf antibody [EP152Y] (ab33899)
(enlarge)


Immunohistochemistry (Paraffin-embedded sections) - B Raf antibody [EP152Y] (ab33899)
Immunohistochemistry (Paraffin-embedded sections) - B Raf antibody [EP152Y] (ab33899)
(enlarge)


Western blot - B Raf antibody [EP152Y] (ab33899)
Western blot - B Raf antibody [EP152Y] (ab33899)
(enlarge)


Product Name 

B Raf antibody [EP152Y]

 

See all B Raf antibodies (15)...

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP152Y] to B Raf

Immunogen 

Synthetic peptide: CGEHNPPSIYLEAYEEYT, corresponding to N terminal amino acids 70-86 of Human B Raf
BLAST 'CGEHNPPSIYLEAYEEYT' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

Flow Cyt, IHC-P, IHC-P, IP, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
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• IHC-P

3 Abreviews

- average rating: 2 stars (out of 5)2 stars (out of 5)2 stars (out of 5)2 stars (out of 5)2 stars (out of 5)

• WB

2 Abreviews

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)


IHC-P
(read Abreview)


IHC-P
(read Abreview)


IHC-P
(read Abreview)


WB
(read Abreview)


Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/50.
IHC-P: 1/250 - 1/500.
IP: 1/50.
WB: 1/1000 - 1/5000. Detects a band of approximately 87 kDa (predicted molecular weight: 85 kDa).

Is unsuitable for ICC.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa cell lysate, human prostate cancer tissue. This antibody also reacts with rat brain tissue.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Cell Biology >> Apoptosis >> Intracellular >> Kinases

Relevance 

The Raf kinases are important intermediates in signal transduction.
Raf protein family members, including A Raf and B Raf, have intrinsic serine/threonine kinase activity. Interaction between Ras proteins and Raf proteins results in Raf-mediated phosphorylation and activation of MEK (also known as MAP kinase kinase). Defects in BRAF are involved in a wide range of cancers.

 

Alternative names B Raf antibody [EP152Y] (ab33899)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

673    (Human)
109880    (Mouse)
114486    (Rat)

GeneCard

    

GC07M139754    (Human)

Omim

    

164757    (Human)

SwissProt

    

P15056    (Human)
Q3USE9    (Mouse)
Q99MC6    (Rat)

Unigene

    

550061    (Human)
99177    (Rat)

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP152Y

Isotype 

IgG

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this B Raf antibody [EP152Y] is on this datasheet. But please do contact us if you would like any reassurance!


See below for B Raf antibody [EP152Y] images, references, products related to ab33899 and other tools.

 

B Raf antibody [EP152Y] images:

Product image  Western blot - B Raf antibody [EP152Y] (ab33899)

Western blot - B Raf antibody [EP152Y] (ab33899)

B Raf antibody [EP152Y] (ab33899) at 1/5000 dilution + HeLa cell lysate

Predicted band size : 85 kDa
Observed band size : 87 kDa (why is the actual band size different from the predicted?)

 

Product image  Immunohistochemistry (Paraffin-embedded sections) - B Raf antibody [EP152Y] (ab33899)

This image shows human prostate cancer stained with ab33899 at 1/250 dilution.

Immunohistochemistry (Paraffin-embedded sections) - B Raf antibody [EP152Y] (ab33899)

This image shows paraffin embedded human prostate cancer tissue sample stained with ab33899 at 1/250 dilution.

 

Product image  Western blot - B Raf antibody [EP152Y] (ab33899)

Western blot - B Raf antibody [EP152Y] (ab33899)

All lanes : B Raf antibody [EP152Y] (ab33899) at 1/1000 dilution

Lane 1 : Lysate prepared from mouse brain
Lane 2 : Lysate prepared from mouse heart
Lane 3 : Lysate prepared from mouse kidney
Lane 4 : Lysate prepared from mouse spleen
Lane 5 : Lysate prepared from rat brain
Lane 6 : Lysate prepared from rat heart
Lane 7 : Lysate prepared from rat kidney
Lane 8 : Lysate prepared from rat spleen


Predicted band size : 85 kDa


Exposure time : 3 minutes

 

References for B Raf antibody [EP152Y] (ab33899)

Specific publication references   This product has been used in:

Pfister S et al. BRAF gene duplication constitutes a mechanism of MAPK pathway activation in low-grade astrocytomas. J Clin Invest 118:1739-1749 (2008). WB; Human. PubMed: 18398503

If you publish research using ab33899 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to B Raf

 

B Raf antibody [EP152Y] - more information

Customer reviews (feedback) regarding B Raf antibody [EP152Y]

Customer FAQs regarding B Raf antibody [EP152Y]

Protocols for B Raf antibody [EP152Y]

Price and availability of products related to B Raf antibody [EP152Y]

 

B Raf antibody [EP152Y] - related products:

Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Slides

Products for Western blot

Loading controls

Lysates

 

Positive Controls for B Raf antibody [EP152Y]

ab29545

  

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

ab5051

  

Prostate tumor (human): adenocarcinoma tissue slides

ab5052

  

Prostate tumor (human): neoplasia tissue slides

 

Compatible Secondaries for B Raf antibody [EP152Y]

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other B Raf Antibodies

See all B Raf antibodies (15)...

 

B Raf antibody [EP152Y] - other tools:

Contact Abcam with a Technical Enquiry about B Raf antibody [EP152Y]

 

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