B Raf antibody [EP152Y] (ab33899)

Product Name 

B Raf antibody [EP152Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP152Y] to B Raf

Immunogen 

Synthetic peptide: CGEHNPPSIYLEAYEEYT, corresponding to N terminal amino acids 70-86 of Human B Raf
BLAST 'CGEHNPPSIYLEAYEEYT' with or

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

Flow Cyt, IHC-P, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/50.
IHC-P: 1/250 - 1/500.
IP: 1/50.
WB: 1/1000 - 1/5000. Detects a band of approximately 87 kDa (predicted molecular weight: 85 kDa).

Is unsuitable for ICC.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa cell lysate, human prostate cancer tissue. This antibody also reacts with rat brain tissue.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Cell Biology >> Apoptosis >> Intracellular >> Kinases

Relevance 

The Raf kinases are important intermediates in signal transduction.
Raf protein family members, including A Raf and B Raf, have intrinsic serine/threonine kinase activity. Interaction between Ras proteins and Raf proteins results in Raf-mediated phosphorylation and activation of MEK (also known as MAP kinase kinase). Defects in BRAF are involved in a wide range of cancers.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP152Y

Isotype 

IgG

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

See below for B Raf antibody [EP152Y] images, references, products related to ab33899 and other tools.

B Raf antibody [EP152Y] images:

References for B Raf antibody [EP152Y] (ab33899)

  This product has been used in:

Pfister S et al. BRAF gene duplication constitutes a mechanism of MAPK pathway activation in low-grade astrocytomas. J Clin Invest 118:1739-1749 (2008). WB; Human. PubMed: 18398503

If you publish research using ab33899 please let us know so that we can cite the reference on this datasheet.

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