Anti-B Raf (phospho T598 + S601) antibody (ab115267)

Overview

• Product name
  Anti-B Raf (phospho T598 + S601) antibody
  See all B Raf primary antibodies
• Description
  Rabbit polyclonal to B Raf (phospho T598 + S601)
• Tested applications
  Suitable for: IHC-P, ICC/IFmore details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  KLH conjugated synthetic phosphopeptide, derived from Human B Raf around pThr598/Ser601.

• Positive control
  • Human testis tissues

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage buffer
  pH: 7.20
  Preservative: 0.09% Sodium azide
  Constituents: 49% PBS, 50% Glycerol
• Concentration information loading...
• Purity
  Immunogen affinity purified
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Cell Biology
  • Apoptosis
  • Intracellular
  • Kinases

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Other

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

  • Cell Biology
  • Cell Cycle
  • Cell differentiation

  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Signal transducers

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

Target

• Function
  Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
• Tissue specificity
  Brain and testis.
• Involvement in disease
  Note=Defects in BRAF are found in a wide range of cancers.
  Defects in BRAF may be a cause of colorectal cancer (CRC) [MIM:114500].
  Defects in BRAF are involved in lung cancer (LNCR) [MIM:211980].
  Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
  Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
  Defects in BRAF are the cause of Noonan syndrome type 7 (NS7) [MIM:613706]. Noonan syndrome is a disorder characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits.
  Defects in BRAF are the cause of LEOPARD syndrome type 3 (LEOPARD3) [MIM:613707]. LEOPARD3 is a disorder characterized by lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and sensorineural deafness.
  Note=A chromosomal aberration involving BRAF is found in pilocytic astrocytomas. A tandem duplication of 2 Mb at 7q34 leads to the expression of a KIAA1549-BRAF fusion protein with a constitutive kinase activity and inducing cell transformation.
• Sequence similarities
  Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
  Contains 1 phorbol-ester/DAG-type zinc finger.
  Contains 1 protein kinase domain.
  Contains 1 RBD (Ras-binding) domain.
• Cellular localization
  Nucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.

• Target information above from: UniProt accession P15056 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 673 Human
  • Omim: 164757 Human
  • SwissProt: P15056 Human
  • Unigene: 550061 Human
• Alternative names
  • FLJ95109 antibody
  • 94 kDa B raf protein antibody
  • B raf 1 antibody

  • B Raf proto oncogene serine threonine protein kinase antibody
  • B Raf proto oncogene, serine/threonine kinase antibody
  • B RAF1 antibody
  • B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody
  • BRAF 1 antibody
  • BRAF antibody
  • BRAF_HUMAN antibody
  • BRAF1 antibody
  • cRmil antibody
  • MGC126806 antibody
  • MGC138284 antibody
  • Murine sarcoma viral (v-raf) oncogene homolog B1 antibody
  • Murine sarcoma viral v raf oncogene homolog B1 antibody
  • NS7 antibody
  • Oncogen BRAF antibody
  • oncogene BRAF1 antibody
  • p94 antibody
  • Proto-oncogene B-Raf antibody
  • Proto-oncogene c-Rmil antibody
  • RAFB 1 antibody
  • RAFB1 antibody
  • RMIL antibody
  • Serine/threonine-protein kinase B-raf antibody
  • v raf murine sarcoma viral oncogene homolog B antibody
  • v raf murine sarcoma viral oncogene homolog B1 antibody
  • v-Raf murine sarcoma viral oncogene homolog B1 antibody

  see all

Protocols

• Immunohistochemistry protocols
• Immunocytochemistry & immunofluorescence protocols