You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

B16 (Mouse melanoma cell line) Nuclear Lysate (ab14638)

    CodeSizePriceAbpointsAvailability
        
    Add to basket
     
    • -
    •   
    •   
    •   
    •  
    •   
    •   
    Updating...

    Overview

    Product name

    B16 (Mouse melanoma cell line) Nuclear Lysate

    General notes

    We recommend aliquoting the extracts into single use fractions and then storing.

    Properties

    Form

    Liquid

    Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Storage buffer

    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9

    Concentration

    Concentration information loading...

    Research areas


    Kits/ Lysates/ Other >> Lysates >> Nuclear Lysates >> Mouse >> Other
    Kits/ Lysates/ Other >> Lysates >> Nuclear Lysates >> Mouse >> Muscle/Skin/Skeleton

    Background

    Transplantable murine melanomas are well-established models for the study of experimental cancer therapies. Melanomas express different tumor-associated Ags, which are potential targets for novel designs of therapeutic cancer vaccines. Melanoma-associated Ags include tyrosinase and tyrosine-related protein (TRP)-1/2 or the MAGE and BAGE class of Ags. Many immunotherapeutic protocols have been tested using the murine B16 melanoma cell line (and its sublines) that originated in the syngeneic C57BL/6 (H-2b) mouse strain. B16 melanoma cells express some of these melanoma-associated Ags, and their growth can be controlled in vivo by different classes of specific or nonspecific immune effector cells. The B16 cell line contains a large amount of PDGF ß-receptor which makes it a suitable positive control for PDGF ß-receptor.

    Applications

    Application notes

    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    References for B16 (Mouse melanoma cell line) Nuclear Lysate (ab14638)

    ab14638 has not yet been referenced specifically in any publications.

    Publishing research using ab14638? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"