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B16 (Mouse melanoma cell line) Nuclear Lysate (ab14638)

Q&A (1)

Overview

  • Product nameB16 (Mouse melanoma cell line) Nuclear Lysate
  • General notesWe recommend aliquoting the extracts into single use fractions and then storing.

Properties

  • BackgroundTransplantable murine melanomas are well-established models for the study of experimental cancer therapies. Melanomas express different tumor-associated Ags, which are potential targets for novel designs of therapeutic cancer vaccines. Melanoma-associated Ags include tyrosinase and tyrosine-related protein (TRP)-1/2 or the MAGE and BAGE class of Ags. Many immunotherapeutic protocols have been tested using the murine B16 melanoma cell line (and its sublines) that originated in the syngeneic C57BL/6 (H-2b) mouse strain. B16 melanoma cells express some of these melanoma-associated Ags, and their growth can be controlled in vivo by different classes of specific or nonspecific immune effector cells. The B16 cell line contains a large amount of PDGF ß-receptor which makes it a suitable positive control for PDGF ß-receptor.

    • Applications

  • Application notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References for B16 (Mouse melanoma cell line) Nuclear Lysate (ab14638)

    ab14638 has not yet been referenced specifically in any publications.

    Publishing research using ab14638 ? Please let us know so that we can cite the reference in this datasheet

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    I wonder if this product could be used as an additive to cultures, besides being used in WB. I suppose it contains a lot of melanocyte specific proteins ?

    Thank you for your enquiry. This product does contain all the nuclear proteins from this melanoma cell line. Moreover, this protein extract is not denatured and most of the proteins are functional. However, we do not have experience or insight of the use ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"