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Anti-B3GALT1 antibody (ab82760)

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Overview

Product name

Anti-B3GALT1 antibody

Description

Rabbit polyclonal to B3GALT1

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Chimpanzee

Immunogen

Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MASKVSCLYV LTVVCWASAL WYLSITRPTS SYTGSKPFSH LTVARKNFTF) of human B3GALT1 (NP_066191).

MASKVSCLYV LTVVCWASAL WYLSITRPTS SYTGSKPFSH LTVARKNFTF

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Fetal heart lysate

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified by peptide affinity chromatography method.

Clonality

Polyclonal

Isotype

IgG

Research areas

Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi

  • Western blot - B3GALT1 antibody (ab82760)Western blot - B3GALT1 antibody (ab82760) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab82760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution. Titre using peptide based assay, 1:312500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

B3GALT1 transfers galactose from UDP-galactose to substrates with a terminal beta-N-acetylglucosamine (beta-GlcNAc) residue. Involved in the biosynthesis of the carbohydrate moieties of glycolipids and glycoproteins. Inactive towards substrates with terminal alpha-N-acetylglucosamine (alpha-GlcNAc) or alpha-N-acetylgalactosamine (alpha-GalNAc) residues.

Cellular localization

Golgi apparatus membrane; Single pass type II membrane protein

Alternative names

  • Beta 1,3 galactosyltransferase 1 antibody
  • beta3Gal T1 antibody
  • UDP Gal:betaGlcNAc beta 1,3 galactosyltransferase, polypeptide 1 antibody

Database links

Anti-B3GALT1 antibody images:

  Western blot - B3GALT1 antibody (ab82760)

Western blot - B3GALT1 antibody (ab82760)

Anti-B3GALT1 antibody (ab82760) at 1 µg/ml + fetal heart lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 38 kDa
Observed band size : 38 kDa

References for Anti-B3GALT1 antibody (ab82760)

ab82760 has not yet been referenced specifically in any publications.

Publishing research using ab82760? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"