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Western blot - B3GNT1 antibody (ab77561)
(enlarge)
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Product Name
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B3GNT1 antibody
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See all B3GNT1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to B3GNT1
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Immunogen
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Synthetic peptide: C-EKHKGFRTFDIE, corresponding to amino acids 348-359 of Human B3GNT1 (NP_006568.2)
BLAST 'C-EKHKGFRTFDIE' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Chk, Cow, Dog, Zfsh
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 46 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human pancreas lysate
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Cellular localization
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Golgi apparatus membrane; Single pass type II membrane protein.
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Research areas
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Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
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Relevance
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B3GNT2 is a member of the beta-1,3-N-acetylglucosaminyltransferase family. It is involved in the initiation and elongation of poly-N-acetyllactosamine chains.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this B3GNT1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for B3GNT1 antibody images, references, products related to ab77561 and other tools.
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B3GNT1 antibody images:
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 Western blot - B3GNT1 antibody (ab77561)
B3GNT1 antibody (ab77561) at 0.1 µg/ml + Human pancreas lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 46 kDa
Observed band size : 46 kDa
Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 hour
Note: The additional 28 kDa band of unknown identity was consistently observed, and the band was successfully blocked by incubation with the immunizing peptide.
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Search PubMed (MEDLINE) for references to B3GNT1
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B3GNT1 antibody - more information
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Customer reviews (feedback) regarding B3GNT1 antibody |
Customer FAQs regarding B3GNT1 antibody |
Protocols for B3GNT1 antibody |
Price and availability of products related to B3GNT1 antibody |
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B3GNT1 antibody - related products:
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B3GNT1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about B3GNT1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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