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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
Overview
Product name
Anti-B3GNT1 antibody
See all B3GNT1 products (2) ...
Description
Goat polyclonal to B3GNT1
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Dog, Zebrafish
Immunogen
Synthetic peptide: C-EKHKGFRTFDIE, corresponding to amino acids 348-359 of Human B3GNT1 (NP_006568.2)
C-EKHKGFRT FDIE
Positive control
Human pancreas lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
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Western blot - B3GNT1 antibody (ab77561)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 0.3 µg/ml.Predicted molecular weight: 46 kDa.
Target
Relevance
B3GNT2 is a member of the beta-1,3-N-acetylglucosaminyltransferase family. It is involved in the initiation and elongation of poly-N-acetyllactosamine chains.
Cellular localization
Golgi apparatus membrane; Single pass type II membrane protein.
Alternative names
- B3GALT7 antibody
- B3Gal T7 antibody
- B3GN T1 antibody
see all
Database links
- Entrez Gene: 10678 Human
- Entrez Gene: 53625 Mouse
- Entrez Gene: 305571 Rat
- Omim: 605581 Human
- SwissProt: Q9NY97 Human
- SwissProt: Q9Z222 Mouse
Anti-B3GNT1 antibody images:
Western blot - B3GNT1 antibody (ab77561)
Anti-B3GNT1 antibody (ab77561) at 0.1 µg/ml + Human pancreas lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 46 kDa
Observed band size : 46 kDa
Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 hour
Note: The additional 28 kDa band of unknown identity was consistently observed, and the band was successfully blocked by incubation with the immunizing peptide.
References for Anti-B3GNT1 antibody (ab77561)
ab77561 has not yet been referenced specifically in any publications.
Publishing research using ab77561? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"