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Anti-B3GNT3 antibody (ab70156)

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Overview

Product name

Anti-B3GNT3 antibody
See all B3GNT3 products (2) ...

Description

Mouse polyclonal to B3GNT3

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Full length B3GNT3 protein (Human) (AAH67423.1)

Positive control

B3GNT3 transfected 293T cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 1X PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Metabolism >> Amino Acids

  • Western blot - B3GNT3 antibody (ab70156)Western blot - B3GNT3 antibody (ab70156) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab70156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: 1/500 - 1/1000. Detects a main band of approximately 40, and fainter bands of 49 and 51 kDa (predicted molecular weight: 43 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

B3GNT3 belongs to the glycosyltransferase 31 family. This enzyme is a type II transmembrane protein and contains a signal anchor that is not cleaved. It prefers the substrates of lacto-N-tetraose and lacto-N-neotetraose and is involved in the biosynthesis of poly-N-acetyllactosamine chains and the biosynthesis of the backbone structure of dimeric sialyl Lewis a. B3GNT3 plays dominant roles in L-selectin ligand biosynthesis, lymphocyte homing and lymphocyte trafficking. It is expressed in colon, jejunum, stomach, esophagus, placenta and trachea.

Cellular localization

Golgi apparatus membrane; Single pass type II membrane protein.

Alternative names

  • B3GAL T8 antibody
  • B3GALT8 antibody
  • B3GN T3 antibody
  • B3GNT 3 antibody
  • Beta 1,3 galactosyltransferase 8 antibody
  • Beta 3 Gal T8 antibody
  • Beta3Gal T8 antibody
  • Beta3GalT8 antibody
  • BGnT 3 antibody
  • BGnT3 antibody
  • Core 1 extending beta 1,3 N acetylglucosaminyltransferase antibody
  • Core1 beta3GlcNAcT antibody
  • HP10328 antibody
  • TMEM3 antibody
  • UDP Gal:beta GlcNAc beta 1,3 galactosyltransferase 8 antibody
  • UDP GlcNAc:betaGal beta 1,3 N acetylglucosaminyltransferase 3 antibody
see all

Database links

Anti-B3GNT3 antibody images:

  Western blot - B3GNT3 antibody (ab70156)

Western blot - B3GNT3 antibody (ab70156)

All lanes : Anti-B3GNT3 antibody (ab70156) at 1/500 dilution

Lane 1 : B3GNT3 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate

Lysates/proteins at 25 µg per lane.

Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size : 43 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Additional bands at : 49 kDa,51 kDa. We are unsure as to the identity of these extra bands.

References for Anti-B3GNT3 antibody (ab70156)

ab70156 has not yet been referenced specifically in any publications.

Publishing research using ab70156? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"