Overview

  • Product nameAnti-B3GNT3 antibody
    See all B3GNT3 primary antibodies
  • Description
    Mouse polyclonal to B3GNT3
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length B3GNT3 protein (Human) (AAH67423.1)

  • Positive control
    • B3GNT3 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab70156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects a main band of approximately 40, and fainter bands of 49 and 51 kDa (predicted molecular weight: 43 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceB3GNT3 belongs to the glycosyltransferase 31 family. This enzyme is a type II transmembrane protein and contains a signal anchor that is not cleaved. It prefers the substrates of lacto-N-tetraose and lacto-N-neotetraose and is involved in the biosynthesis of poly-N-acetyllactosamine chains and the biosynthesis of the backbone structure of dimeric sialyl Lewis a. B3GNT3 plays dominant roles in L-selectin ligand biosynthesis, lymphocyte homing and lymphocyte trafficking. It is expressed in colon, jejunum, stomach, esophagus, placenta and trachea.
    • Cellular localizationGolgi apparatus membrane; Single pass type II membrane protein.
    • Database links
    • Alternative names
      • B3GAL T8 antibody
      • B3GALT8 antibody
      • B3GN T3 antibody
      • B3GNT 3 antibody
      • Beta 1,3 galactosyltransferase 8 antibody
      • Beta 3 Gal T8 antibody
      • Beta3Gal T8 antibody
      • Beta3GalT8 antibody
      • BGnT 3 antibody
      • BGnT3 antibody
      • Core 1 extending beta 1,3 N acetylglucosaminyltransferase antibody
      • Core1 beta3GlcNAcT antibody
      • HP10328 antibody
      • TMEM3 antibody
      • UDP Gal:beta GlcNAc beta 1,3 galactosyltransferase 8 antibody
      • UDP GlcNAc:betaGal beta 1,3 N acetylglucosaminyltransferase 3 antibody
      see all

    Anti-B3GNT3 antibody images

    • All lanes : Anti-B3GNT3 antibody (ab70156) at 1/500 dilution

      Lane 1 : B3GNT3 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 43 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 49 kDa,51 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-B3GNT3 antibody (ab70156)

    ab70156 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (SU.86.86 (pancreatic carcinoma - liver metastasis))
    Permeabilization Yes - 0.2% Triton X-100
    Specification SU.86.86 (pancreatic carcinoma - liver metastasis)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative Formaldehyde
    Username

    Dr. Armen Petrosyan

    Verified customer

    Submitted Jul 27 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"