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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
Overview
Product name
Anti-B3GNT5 antibody
See all B3GNT5 products (2) ...
Description
Mouse polyclonal to B3GNT5
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length human B3GNT5 protein (NP_114436).
Positive control
B3GNT5 transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
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Western blot - B3GNT5 antibody (ab69272)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab69272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
Target
Relevance
B3GNT5 is a beta 1,3 N acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. It has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc(4)Cer; paragloboside), resulting in the synthesis of Lc(3)Cer and neolactopentaosylceramide (nLc(5)Cer), respectively.
Cellular localization
Golgi Apparatus membrane; Single-pass type II membrane protein
Alternative names
- B3GN-T5 antibody
- B3GNT5 antibody
- Beta 1,3 N acetyglucosaminyltransferase Lc3 synthase antibody
see all
Database links
- Entrez Gene: 84002 Human
- SwissProt: Q9BYG0 Human
Anti-B3GNT5 antibody images:
Western blot - B3GNT5 antibody (ab69272)
All lanes : Anti-B3GNT5 antibody (ab69272) at 1/500 dilution
Lane 1 : B3GNT5 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 44 kDa
Observed band size : 44 kDa
References for Anti-B3GNT5 antibody (ab69272)
ab69272 has not yet been referenced specifically in any publications.
Publishing research using ab69272? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"