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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
Overview
Product name
Anti-B4GALT5 antibody
See all B4GALT5 products (3) ...
Description
Mouse monoclonal to B4GALT5
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment, corresponding to amino acids 48-152 of Human B4GALT5
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Signal Transduction >> Metabolism >> Energy Metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Golgi
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Western blot - B4GALT5 antibody (ab56558)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a dilution 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 45 kDa).
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
Tissue specificity
Ubiquitously expressed.
Pathway
Protein modification; protein glycosylation.
Sequence similarities
Belongs to the glycosyltransferase 7 family.
Cellular localization
Golgi apparatus > Golgi stack membrane. Trans cisternae of Golgi stack.
Target information above from: UniProt accessionO43286
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 4-galactosyltransferase 5 antibody
- 4-GalT II antibody
- 4-GalTase 5 antibody
see all
Database links
- Entrez Gene: 9334 Human
- Omim: 604016 Human
- SwissProt: O43286 Human
- Unigene: 370487 Human
Anti-B4GALT5 antibody images:
Western blot - B4GALT5 antibody (ab56558)
Anti-B4GALT5 antibody (ab56558) at 5 µg/ml + partial 0.2 µg/lane of partial recombinant B4GALT5 protein with a tag
Secondary
Goat anti mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
Predicted band size : 45 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
References for Anti-B4GALT5 antibody (ab56558)
ab56558 has not yet been referenced specifically in any publications.
Publishing research using ab56558? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"