Anti-B7H4 antibody (ab77281)

Overview

• Product nameAnti-B7H4 antibodySee all B7H4 primary antibodies ...
• Description
  Goat polyclonal to B7H4
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig, Chimpanzee, Macaque Monkey
• Immunogen

  Synthetic peptide: C-SKGKGNANLEYK, corresponding to amino acids 135-146 of Human B7H4 (NP_078902.2)

  Run BLAST with Run BLAST with
• Positive control Human Lung, Spleen and Thymus lysates

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Cell cycle inhibitors
  • Other

  • Immunology
  • Innate Immunity
  • Cytokines
  • Interleukins

  • Immunology
  • Adaptive Immunity
  • Regulatory T Cells

  • Cell Biology
  • Cell Cycle
  • Cell Cycle Inhibitors
  • Other

Applications

Target

• FunctionNegatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.
• Tissue specificityOverexpressed in breast, ovarian, endometrial, renal cell (RCC) and non-small-cell lung cancers (NSCLC). Expressed on activated T- and B-cells, monocytes and dendritic cells, but not expressed in most normal tissues (at protein level). Widely expressed, including in kidney, liver, lung, ovary, placenta, spleen and testis.
• Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family.
  Contains 2 Ig-like V-type (immunoglobulin-like) domains.
• Post-translational
  modificationsN-glycosylated.
• Cellular localizationCell membrane. Expressed at the cell surface. A soluble form has also been detected.

Target information above from: UniProt accession Q7Z7D3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 539919 Cow
  • Entrez Gene: 483140 Dog
  • Entrez Gene: 79679 Human
  • Entrez Gene: 242122 Mouse
  • Entrez Gene: 295322 Rat
  • Omim: 608162 Human
  • SwissProt: Q7Z7D3 Human
  • SwissProt: Q7TSP5 Mouse

  • SwissProt: Q501W4 Rat
  • Unigene: 546434 Human
  • Unigene: 137467 Mouse
  • Unigene: 160956 Rat

  see all

• Alternative names
  B7 family member, H4 antibodyB7 H4 antibodyB7 superfamily member 1 antibody

  B7-H4 antibodyB7h.5 antibodyB7h4 antibodyB7S1 antibodyB7x antibodyBC032925 antibodyImmune costimulatory protein B7-H4 antibodyImmune costimulatory protein B7H4 antibodyMGC41287 antibodyPRO1291 antibodyProtein B7S1 antibodyRP11 229A19.4 antibodyT cell costimulatory molecule B7x antibodyT-cell costimulatory molecule B7x antibodyV set domain-containing T cell activation inhibitor 1 antibodyV-set domain-containing T-cell activation inhibitor 1 antibodyVCTN1 antibodyVtcn1 antibodyVTCN1_HUMAN antibody

  see all

Anti-B7H4 antibody images

• 

  Western blot - Anti-B7H4 antibody (ab77281)
  Anti-B7H4 antibody (ab77281) at 0.5 µg/ml + Human Pancrease lysate in RIPA Buffer at 35 µg
  developed using the ECL technique
  
  Predicted band size : 31 kDa
  Observed band size : 28 kDa (why is the actual band size different from the predicted?)