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Products:Signal Transduction >> Metabolism >> Lipid metabolism
Overview
Product name
Anti-BAAT antibody
See all BAAT products (3) ...
Description
Rabbit polyclonal to BAAT
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Dog
Immunogen
Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (IQLTATPVSA LVDEPVHIRA TGLIPFQMVS FQASLEDENG DMFYSQAHYR) of human BAAT (NP_001692).
IQLTATPVSA LVDEPVHIRA TGLIPFQMVS FQASLEDENG DMFYSQAHYR
Positive control
Human fetal liver lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified by peptide affinity chromatography method.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
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Western blot - BAAT antibody (ab83882)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab83882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa.
ELISA titre using peptide based assay, 1:62500.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
BAAT (bile acid Coenzyme A: amino acid N-acyltransferase) is involved in bile acid metabolism. In liver hepatocytes BAAT catalyzes the transfer of the bile acid moiety from the acyl-CoA thioester to glycine and taurine, before excretion into bile canaliculi. This is the second step in the formation of bile acid-amino acid conjugates. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. BAAT may also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids.
Cellular localization
Cytoplasmic
Alternative names
- FLJ20300 antibody
- BACAT antibody
- BAT antibody
see all
Database links
- Entrez Gene: 570 Human
- Entrez Gene: 12012 Mouse
- Entrez Gene: 29725 Rat
- Omim: 602938 Human
- SwissProt: Q14032 Human
- SwissProt: Q91X34 Mouse
- SwissProt: Q63276 Rat
- Unigene: 284712 Human
Anti-BAAT antibody images:
Western blot - BAAT antibody (ab83882)
Anti-BAAT antibody (ab83882) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal liver lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 46 kDa
Observed band size : 46 kDa
References for Anti-BAAT antibody (ab83882)
ab83882 has not yet been referenced specifically in any publications.
Publishing research using ab83882? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"