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Overview

  • Product nameAnti-BAAT antibodySee all BAAT primary antibodies ...
  • Description
    Rabbit polyclonal to BAAT
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (IQLTATPVSA LVDEPVHIRA TGLIPFQMVS FQASLEDENG DMFYSQAHYR) of human BAAT (NP_001692).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlHuman fetal liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa.

    ELISA titre using peptide based assay, 1:62500.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionInvolved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids). May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs.
    • Tissue specificityExpressed in liver, gallbladder mucosa and pancreas.
    • Involvement in diseaseDefects in BAAT are involved in familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.
    • Sequence similaritiesBelongs to the C/M/P thioester hydrolase family.
    • Cellular localizationCytoplasm.

      • Target information above from: UniProt accession Q14032 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        FLJ20300 antibodyBAAT antibodyBAAT_HUMAN antibody
        BACAT antibodyBAT antibodyBile acid CoA: amino acid N-acyltransferase (glycine N-choloyltransferase) antibodyBile acid CoA:amino acid N acyltransferase antibodyBile acid Coenzyme A amino acid N acyltransferase glycine N choloyltransferase antibodyBile acid Coenzyme A: amino acid N acyltransferase antibodyBile acid-CoA:amino acid N-acyltransferase antibodyGlycine N choloyltransferase antibodyGlycine N-choloyltransferase antibodyLong chain fatty acyl CoA hydrolase antibodyLong-chain fatty-acyl-CoA hydrolase antibodyMGC104432 antibody
      see all

    Anti-BAAT antibody images

    • Western blot - BAAT antibody (ab83882)
      Western blot - BAAT antibody (ab83882)
      Anti-BAAT antibody (ab83882) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal liver lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size : 46 kDa
      Observed band size : 46 kDa

    References for Anti-BAAT antibody (ab83882)

    This product has been referenced in:
    • Aleksunes LM  et al. Repression of hepatobiliary transporters and differential regulation of classic and alternative bile acid pathways in mice during pregnancy. Toxicol Sci 130:257-68 (2012). WB ; Mouse . Read more (PubMed: 22903823) »

    See 1 Publication for this product

    Publishing research using ab83882 ? Please let us know so that we can cite the reference in this datasheet

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    Western blot abreview for Anti-BAAT antibody

     Good
    Abreviews
    Application Western blot
    Sample Mouse Tissue lysate - whole (liver)
    Loading amount 50 µg
    Specification liver
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    Verified customer

    Submitted Jun 23 2012

    The WB results of ab83882 and ab97884 used in mouse liver tissues has been attached here. I also sent the results to Abreview.

    Thank you for your reply and for submitting your Abreviews. The discount codes I had sent you are now active and can each be used to receive a free antibody on a future order. If you are interested in testing any other antibodies, please let me know and I...

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    I would like to test ab83882 and ab97884 for use on mouse samples.

    For ab83882: DISCOUNT CODE: ***
    For ab97884: DISCOUNT CODE: ***
    Expiration date: October 5th, 2012

    I am very pleased to hear you would like to accept our offer and test ab83882 and ab97884 in mouse.Each code will give you: 1 free primar...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"