Feedback Form

Western blot - BAAT antibody (ab83882)
Western blot - BAAT antibody (ab83882)
(enlarge)


Product Name 

BAAT antibody

 

See all BAAT antibodies (2)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to BAAT

Immunogen 

Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (IQLTATPVSA LVDEPVHIRA TGLIPFQMVS FQASLEDENG DMFYSQAHYR) of human BAAT (NP_001692).
BLAST 'IQLTATPVSA LVDEPVHIRA TGLIPFQMVS FQASLEDENG DMFYSQAHYR ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews

Submit an Abreview

Be the first to review this antibody and earn 220 Abpoints.


Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa.

ELISA titre using peptide based assay, 1:62500.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human fetal liver lysate

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism

Relevance 

BAAT (bile acid Coenzyme A: amino acid N-acyltransferase) is involved in bile acid metabolism. In liver hepatocytes BAAT catalyzes the transfer of the bile acid moiety from the acyl-CoA thioester to glycine and taurine, before excretion into bile canaliculi. This is the second step in the formation of bile acid-amino acid conjugates. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. BAAT may also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids.

 

BAAT antibody (ab83882)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

570    (Human)

GeneCard

    

GC09M103162    (Human)

Omim

    

602938    (Human)

SwissProt

    

Q14032    (Human)

Unigene

    

284712    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Purification notes 

Purified by peptide affinity chromatography method.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BAAT antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for BAAT antibody images, references, products related to ab83882 and other tools.

 

BAAT antibody images:

Product image  Western blot - BAAT antibody (ab83882)

Western blot - BAAT antibody (ab83882)

BAAT antibody (ab83882) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal liver lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 46 kDa
Observed band size : 46 kDa

 
 

Search PubMed (MEDLINE) for references to BAAT

 

BAAT antibody - more information

Customer reviews (feedback) regarding BAAT antibody

Customer FAQs regarding BAAT antibody

Protocols for BAAT antibody

Price and availability of products related to BAAT antibody

 

BAAT antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for BAAT antibody

ab29876

  

Liver (Human) Cytoplasmic Lysate - fetal normal tissue

 

Compatible Secondaries for BAAT antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other BAAT Antibodies

See all BAAT antibodies (2)...

 

BAAT antibody - other tools:

Contact Abcam with a Technical Enquiry about BAAT antibody

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.