Overview

  • Product nameAnti-BAAT antibodySee all BAAT primary antibodies ...
  • Description
    Rabbit polyclonal to BAAT
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (IQLTATPVSA LVDEPVHIRA TGLIPFQMVS FQASLEDENG DMFYSQAHYR) of human BAAT (NP_001692).

  • Positive control
    • Human fetal liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionInvolved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids). May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs.
  • Tissue specificityExpressed in liver, gallbladder mucosa and pancreas.
  • Involvement in diseaseDefects in BAAT are involved in familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.
  • Sequence similaritiesBelongs to the C/M/P thioester hydrolase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ20300 antibody
    • BAAT antibody
    • BAAT_HUMAN antibody
    • BACAT antibody
    • BAT antibody
    • Bile acid CoA: amino acid N-acyltransferase (glycine N-choloyltransferase) antibody
    • Bile acid CoA:amino acid N acyltransferase antibody
    • Bile acid Coenzyme A amino acid N acyltransferase glycine N choloyltransferase antibody
    • Bile acid Coenzyme A: amino acid N acyltransferase antibody
    • Bile acid-CoA:amino acid N-acyltransferase antibody
    • Glycine N choloyltransferase antibody
    • Glycine N-choloyltransferase antibody
    • Long chain fatty acyl CoA hydrolase antibody
    • Long-chain fatty-acyl-CoA hydrolase antibody
    • MGC104432 antibody
    see all

Anti-BAAT antibody images

  • Anti-BAAT antibody (ab83882) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 46 kDa

References for Anti-BAAT antibody (ab83882)

This product has been referenced in:
  • Aleksunes LM  et al. Repression of hepatobiliary transporters and differential regulation of classic and alternative bile acid pathways in mice during pregnancy. Toxicol Sci 130:257-68 (2012). WB ; Mouse . Read more (PubMed: 22903823) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Loading amount 50 µg
Specification liver
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Xia Wen

Verified customer

Submitted Jun 23 2012

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Expiration date: October 5th, 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"