Anti-BAAT antibody (ab83882)
- Product nameAnti-BAAT antibodySee all BAAT primary antibodies ...
- DescriptionRabbit polyclonal to BAAT
- Tested applicationsWB more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Dog
Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (IQLTATPVSA LVDEPVHIRA TGLIPFQMVS FQASLEDENG DMFYSQAHYR) of human BAAT (NP_001692).
- Positive controlHuman fetal liver lysate
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesPurified by peptide affinity chromatography method.
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab83882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA titre using peptide based assay, 1:62500.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- FunctionInvolved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids). May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs.
- Tissue specificityExpressed in liver, gallbladder mucosa and pancreas.
- Involvement in diseaseDefects in BAAT are involved in familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.
- Sequence similaritiesBelongs to the C/M/P thioester hydrolase family.
- Cellular localizationCytoplasm.
- FLJ20300 antibodyBAAT antibodyBAAT_HUMAN antibody
- BACAT antibodyBAT antibodyBile acid CoA: amino acid N-acyltransferase (glycine N-choloyltransferase) antibodyBile acid CoA:amino acid N acyltransferase antibodyBile acid Coenzyme A amino acid N acyltransferase glycine N choloyltransferase antibodyBile acid Coenzyme A: amino acid N acyltransferase antibodyBile acid-CoA:amino acid N-acyltransferase antibodyGlycine N choloyltransferase antibodyGlycine N-choloyltransferase antibodyLong chain fatty acyl CoA hydrolase antibodyLong-chain fatty-acyl-CoA hydrolase antibodyMGC104432 antibody
Anti-BAAT antibody images
Anti-BAAT antibody (ab83882) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal liver lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 46 kDa
Observed band size : 46 kDa
References for Anti-BAAT antibody (ab83882)
This product has been referenced in:
- Aleksunes LM et al. Repression of hepatobiliary transporters and differential regulation of classic and alternative bile acid pathways in mice during pregnancy. Toxicol Sci 130:257-68 (2012). WB ; Mouse . Read more (PubMed: 22903823) »