Recombinant Anti-BRAT1 antibody [EPR13753-43] - C-terminal (ab181868)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13753-43] to BRAT1 - C-terminal
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-BRAT1 antibody [EPR13753-43] - C-terminal
See all BRAT1 primary antibodies -
Description
Rabbit monoclonal [EPR13753-43] to BRAT1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, 293 and Jurkat cell lysates; 293 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13753-43 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/2000. Detects a band of approximately 75 kDa (predicted molecular weight: 88 kDa).
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IP |
1/50.
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Notes |
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Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/2000. Detects a band of approximately 75 kDa (predicted molecular weight: 88 kDa). |
IP
1/50. |
Target
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Function
Required for activation of ATM following ionizing radiation. May act by regulating dephosphorylation of ATM. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Contains 2 HEAT repeats. -
Cellular localization
Nucleus. Present at double strand breaks (DSBs) following ionizing radiation treatment. - Information by UniProt
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Database links
- Entrez Gene: 221927 Human
- Omim: 614506 Human
- SwissProt: Q6PJG6 Human
- Unigene: 520623 Human
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Alternative names
- BAAT 1 antibody
- BAAT1 antibody
- BRAT 1 antibody
see all
Images
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All lanes : Anti-BRAT1 antibody [EPR13753-43] - C-terminal (ab181868) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 88 kDa -
Intracellular flow cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%) labeling BRAT1with ab181868 at 1/100 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab181868 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181868 has not yet been referenced specifically in any publications.