Overview

  • Product nameAnti-BAAT1 antibodySee all BAAT1 primary antibodies ...
  • Description
    Rabbit polyclonal to BAAT1
  • Tested applicationsICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human BAAT1.

    (Peptide available as ab35881.)

  • Positive control
    • ab35882 gives a positive result in Western Blot against the following whole cell lysates: HeLa, Jurkat, A431 and HEK 293.

Properties

Applications

Our Abpromise guarantee covers the use of ab35882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).Can be blocked with Human BAAT1 peptide (ab35881).

Target

  • FunctionRequired for activation of ATM following ionizing radiation. May act by regulating dephosphorylation of ATM.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesContains 2 HEAT repeats.
  • Cellular localizationNucleus. Present at double strand breaks (DSBs) following ionizing radiation treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAAT 1 antibody
    • BAAT1 antibody
    • BRAT 1 antibody
    • brat1 antibody
    • BRAT1_HUMAN antibody
    • BRCA1-associated ATM activator 1 antibody
    • BRCA1-associated protein required for ATM activation protein 1 antibody
    • BRCA1-associated protein required for ATM activation-1 antibody
    • C7orf27 antibody
    • chromosome 7 open reading frame 27 antibody
    • HEAT repeat containing protein C7orf27 antibody
    • MGC22916 antibody
    see all

Anti-BAAT1 antibody images

  • All lanes : Anti-BAAT1 antibody (ab35882) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 88 kDa
    Observed band size : 88 kDa
    Additional bands at : 110 kDa (possible cross reactivity),170 kDa (possible cross reactivity).
  • ICC/IF image of ab35882 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab35882, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

References for Anti-BAAT1 antibody (ab35882)

ab35882 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (BJ)
Loading amount 20 µg
Specification BJ
Gel Running Conditions Reduced Non-Denaturing (Native) (7.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1%
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Submitted May 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"