Overview
- Product nameAnti-BAAT1 antibodySee all BAAT1 primary antibodies ...
- DescriptionRabbit polyclonal to BAAT1
- Tested applicationsICC/IF, WB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human BAAT1.
(Peptide available as ab35881.)
- Positive controlab35882 gives a positive result in Western Blot against the following whole cell lysates: HeLa, Jurkat, A431 and HEK 293.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab35882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use a concentration of 1 µg/ml. |
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).Can be blocked with BAAT1 peptide (ab35881). |
Target
- FunctionRequired for activation of ATM following ionizing radiation. May act by regulating dephosphorylation of ATM.
- Tissue specificityUbiquitously expressed.
- Sequence similaritiesContains 2 HEAT repeats.
- Cellular localizationNucleus. Present at double strand breaks (DSBs) following ionizing radiation treatment.
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Database links
- Entrez Gene: 221927 Human
- Entrez Gene: 231841 Mouse
- SwissProt: Q6PJG6 Human
- SwissProt: Q8C3R1 Mouse
- SwissProt: D3ZUM1 Rat
- Unigene: 520623 Human
- Unigene: 271932 Mouse
Target information above from: UniProt accession
Q6PJG6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- BAAT 1 antibodyBAAT1 antibodyBRAT 1 antibody
- brat1 antibodyBRAT1_HUMAN antibodyBRCA1-associated ATM activator 1 antibodyBRCA1-associated protein required for ATM activation protein 1 antibodyBRCA1-associated protein required for ATM activation-1 antibodyC7orf27 antibodychromosome 7 open reading frame 27 antibodyHEAT repeat containing protein C7orf27 antibodyMGC22916 antibody
see all
Anti-BAAT1 antibody images
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Western blot - BAAT1 antibody (ab35882)All lanes : Anti-BAAT1 antibody (ab35882) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 :A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lane 4 :HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 88 kDa
Observed band size : 88 kDa
Additional bands at : 110 kDa (possible cross reactivity),170 kDa (possible cross reactivity). -
Immunocytochemistry/ Immunofluorescence - BAAT1 antibody (ab35882)ICC/IF image of ab35882 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab35882, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-BAAT1 antibody (ab35882)
ab35882 has not yet been referenced specifically in any publications.
