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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Proteases
Overview
Product name
Anti-BACE1 antibody - Catalytic domain
See all BACE1 products (14) ...
Description
Rabbit polyclonal to BACE1 - Catalytic domain
Specificity
ab38891 recognizes all four of the splice variants of BACE1.
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Synthetic peptide based on catalytic domain of Human BACE1.
(Peptide available as ab41285.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab38891 has been peptide-affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Aspartic protease >> BACEs
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Proteases
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Immunocytochemistry/ Immunofluorescence - BACE1 antibody - Catalytic domain (ab38891)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab38891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
- 1 Image
ICC/IF
Application notes
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/1000 - 1/5000. Detects a band of approximately 64 kDa (predicted molecular weight: 56 kDa). Notes: 1/1,000 when using colorimetric substrates such as BCIP/NBT, and 1/5,000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
Tissue specificity
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
Sequence similarities
Belongs to the peptidase A1 family.
Domain
The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.
Post-translational
modifications
Glycosylated.
Cellular localization
Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
Target information above from: UniProt accessionP56817
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- APP beta secretase antibody
- APP beta secretase antibody
- Asp 2 antibody
see all
Database links
- Entrez Gene: 23621 Human
- Entrez Gene: 29392 Rat
- Omim: 604252 Human
- SwissProt: P56817 Human
- SwissProt: P56819 Rat
- Unigene: 504003 Human
- Unigene: 207201 Rat
Anti-BACE1 antibody - Catalytic domain images:
Immunocytochemistry/ Immunofluorescence - BACE1 antibody - Catalytic domain (ab38891)
ICC/IF image of ab38891 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38891, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References for Anti-BACE1 antibody - Catalytic domain (ab38891)
ab38891 has not yet been referenced specifically in any publications.
Publishing research using ab38891? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"