![Western blot - BACE1 antibody [EPR3956] (ab108394) image](#BACE1-Primary-antibodies-ab108394-1.jpg)
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Product name
Anti-BACE1 antibody [EPR3956]
See all BACE1 products (14) ...
Description
Rabbit monoclonal [EPR3956] to BACE1
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to residues in Human BACE1.
Positive control
Fetal brain, SH-SY5Y, Mouse brain and Rat brain lysates
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR3956
Isotype
IgG1
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Aspartic protease >> BACEs
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Proteases
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Western blot - BACE1 antibody [EPR3956] (ab108394)
![Western blot - BACE1 antibody [EPR3956] (ab108394) image](/ps/datasheet/images/108/ab108394/BACE1-Primary-antibodies-ab108394-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab108394 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IP
IP: 1/10 - 1/100.IP: 1/10 - 1/100.
Application notes
Is unsuitable for ,Flow Cyt,ICC or IHC-P.
Target
Function
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
Tissue specificity
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
Sequence similarities
Belongs to the peptidase A1 family.
Domain
The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.
Post-translational
modifications
Glycosylated.
Cellular localization
Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
Target information above from: UniProt accessionP56817
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- APP beta secretase antibody
- Asp 2 antibody
- ASP2 antibody
see all
Database links
- Entrez Gene: 23621 Human
- Entrez Gene: 23821 Mouse
- Entrez Gene: 29392 Rat
- Omim: 604252 Human
- SwissProt: P56817 Human
- SwissProt: P56818 Mouse
- SwissProt: P56819 Rat
- Unigene: 504003 Human
see all
Anti-BACE1 antibody [EPR3956] images:
Western blot - BACE1 antibody [EPR3956] (ab108394)
All lanes : Anti-BACE1 antibody [EPR3956] (ab108394) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : SH-SY5Y lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 56 kDa
References for Anti-BACE1 antibody [EPR3956] (ab108394)
ab108394 has not yet been referenced specifically in any publications.
Publishing research using ab108394? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"