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Related products
- Proteins and Peptides
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab2077 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
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Called because product was giving non specific bands |
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ANSWER: |
Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued the free of charge blocking peptide for this product with the order number I have also included our protocol for blocking with the immunizing peptide for your review. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research. |
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Dear Tanya, Thank you for the previous e-mail concerning the blocking peptide. I have just tried the peptide and the antibody at 1:1 ratio and I am glad to say that preliminary results are positive. It appears that the peptide has blocked the antibody "exceptionally well" even though I was sceptical. The result is what we have been looking for and we will be retesting the experiment two more times with every confidence of it working. However, we were looking at 3 bands of interest and one of which in this experiment appears to be non specific binding. To be certain of this we would very much appreciate a picture of what this antibody detects in human brain lysate as described in the product data sheet. The spurious band is approximately 65kDa in size but not 70kDa. This difference is very important. Our next step will be to test another peptide we have been interested in and we will be purchasing this from abcam as well. Thank you very much again for your help and advice. Best regards,A very satisfied customer. |
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ANSWER: |
Tanya was away on vacation when we received your e-mail requesting a WB image. I am sorry it has taken so long to generate this image, but I hope that the image I just forwarded as a word document will help you to identify the correct band. |
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Will this antibody react with rat BACE and is the 3T3 cell lysate WB in the datasheet from transfected or untransfected cells? |
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ANSWER: |
Thank you for your interest in our antibody products. Unfortunatly, this antibody has only been tested in mouse and human. also, our lysates are all from untransfected cells. Best regards,
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Do you have any plans to produce an antibody to the pro-domain of BACE?
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ANSWER: |
No. We do not plan to make antibodies to the pro-domain of BACE, however, we offer custom antibody production at greatly reduced prices. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
