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- Secondary antibodies
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Product name
Anti-BACE1 antibody
See all BACE1 products (14) ...
Description
Rabbit polyclonal to BACE1
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S498 of human BACE1.
Positive control
Mouse cerebellum tissue lysates
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Aspartic protease >> BACEs
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Proteases
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Western blot - BACE1 antibody (ab76019)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76019 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: 1/1000.
WB: 1/50 - 1/100. Detects bands of approximately 28, 34, 56 and 60 kDa (predicted molecular weight: 56 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
Tissue specificity
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
Sequence similarities
Belongs to the peptidase A1 family.
Domain
The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.
Post-translational
modifications
Glycosylated.
Cellular localization
Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
Target information above from: UniProt accessionP56817
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- APP beta secretase antibody
- Asp 2 antibody
- ASP2 antibody
see all
Database links
- Entrez Gene: 23621 Human
- Entrez Gene: 23821 Mouse
- Omim: 604252 Human
- SwissProt: P56817 Human
- SwissProt: P56818 Mouse
- Unigene: 504003 Human
- Unigene: 24044 Mouse
Anti-BACE1 antibody images:
Western blot - BACE1 antibody (ab76019)
Anti-BACE1 antibody (ab76019) at 1/50 dilution + Mouse cerebellum tissue lysates at 35 µg
Predicted band size : 56 kDa
Observed band size : 56 kDa
Additional bands at : 28 kDa,34 kDa,60 kDa. We are unsure as to the identity of these extra bands.
References for Anti-BACE1 antibody (ab76019)
ab76019 has not yet been referenced specifically in any publications.
Publishing research using ab76019? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"