Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Overview
Product name
Anti-BACH1.3 antibody
See all BACH1.3 products (5) ...
Description
Mouse monoclonal to BACH1.3
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: HLAKGFWSDI CSTDTPCQMQ LSPAVAKDGS EQISQKRSEC PWLGIRISES PEPGQRTFTT LSSVNCPFIS TLSTEGCSSN LEIGNDDYVS EPQQEPC, corresponding to amino acids 396-493 of Human BACH1.3
HLAKGFWSDI CSTDTPCQMQ LSPAVAKDGS EQISQKRSEC PWLGIRISES PEPGQRTFTT LSSVNCPFIS TLSTEGCSSN LEIGNDDYVS EPQQEPC
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 82 kDa.
IHC-P: Use at an assay dependent dilution. (PubMed: 21541331)
Target
Function
Transcriptional regulator that acts as repressor or activator. Binds, in-vitro, to NF-E2 binding sites. Play important roles in coordinating transcription activation and repression by MAFK.
Sequence similarities
Belongs to the bZIP family. CNC subfamily.
Contains 1 BTB (POZ) domain.
Contains 1 bZIP domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO14867
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BACH 1 antibody
- BACH 1.3 antibody
- BACH1 antibody
see all
Database links
- Entrez Gene: 571 Human
- Omim: 602751 Human
- SwissProt: O14867 Human
- Unigene: 154276 Human
- Unigene: 721915 Human
Anti-BACH1.3 antibody images:
Western blot - BACH1.3 antibody (ab54814)
Anti-BACH1.3 antibody (ab54814) at 5 µg/ml + 0.2 µg/lane of recombinant protein with a tag
Secondary
Goat anti mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
Predicted band size : 82 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Note: The difference between predicted and actual band sizes may be due to the fact that ab54814 was tested against a recombinant partial protein, and not the full length protein.
Western blot - BACH1.3 antibody (ab54814)
Anti-BACH1.3 antibody (ab54814) at 5 µg/ml +
50 µg/lane of
NIH/3T3 cell lysate
Secondary
Goat anti mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size : 82 kDa
Observed band size : 82 kDa
ELISA - BACH1.3 antibody (ab54814)
ab54814, staining recombinant tagged BACH1.3 by capture ELISA. The detection limit is approximately 0.03ng/ml as a capture antibody.
References for Anti-BACH1.3 antibody (ab54814)
This product has been referenced in:
- Du ZMet al. Upregulation of MiR-155 in Nasopharyngeal Carcinoma is Partly Driven by LMP1 and LMP2A and Downregulates a Negative Prognostic Marker JMJD1A. PLoS One 6:e19137 (2011). IHC-P; Human.Read more (PubMed: 21541331) »
See 1 publication for this product
Publishing research using ab54814? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"