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All lanes : Anti-BACH1/BRIP1 antibody (ab3502) at 1/1000 dilution
Lane 1 : 293 whole cell lysate
Lane 2 : 293 whole cell lysate with peptide immunogen
Lysates/proteins at 35 µg per lane.
Secondary
IRDye 800 conjugated Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 140 kDa
Observed band size : 105 kDa (why is the actual band size different from the predicted?)
Detects a band at 105kD, that is blocked by the immunizing peptide.
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