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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab3502 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - BACH1 antibody (ab3502)
All lanes : Anti-BACH1/BRIP1 antibody (ab3502) at 1/1000 dilution
Lane 1 : 293 whole cell lysate
Lane 2 : 293 whole cell lysate with peptide immunogen
Lysates/proteins at 35 µg per lane.
Secondary
IRDye 800 conjugated Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 140 kDa
Observed band size : 105 kDa (why is the actual band size different from the predicted?)
Detects a band at 105kD, that is blocked by the immunizing peptide.
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