Anti-BACH1/BRIP1 antibody (ab3502)

Overview

• Product nameAnti-BACH1/BRIP1 antibodySee all BACH1/BRIP1 primary antibodies ...
• Description
  Rabbit polyclonal to BACH1/BRIP1
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide: SKDFTNNDMNQGT, corresponding to amino acids 92-104 of Human BACH 1.

  Run BLAST with Run BLAST with
• General notesExpiration date is one (1) year from date of opening.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Sodium Azide
  Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThis is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • BRCT Domain Proteins

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • Fanconi's Anemia

Applications

Target

• FunctionDNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1.
• Tissue specificityUbiquitously expressed, with highest levels in testis.
• Involvement in diseaseDefects in BRIP1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
  Defects in BRIP1 are the cause of Fanconi anemia complementation group J (FANCJ) [MIM:609054]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
• Sequence similaritiesBelongs to the DEAD box helicase family. DEAH subfamily.
  Contains 1 helicase ATP-binding domain.
• Domain4Fe-4S iron-sulfur-binding is required for helicase activity (PubMed:20639400).
• Post-translational
  modificationsPhosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q9BX63 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 83990 Human
  • Omim: 605882 Human
  • SwissProt: Q9BX63 Human
  • Unigene: 128903 Human

• Alternative names
  ATP dependent RNA helicase BRIP1 antibodyATP-dependent RNA helicase BRIP1 antibodyBACH 1 antibody

  BACH 1 antibodyBRAC 1 Associated C Terminal Helicase 1 antibodyBRCA 1 Interacting Protein 1 antibodyBRCA1 binding helicase like protein BACH1 antibodyBRCA1 interacting protein C terminal helicase 1 antibodyBRCA1-associated C-terminal helicase 1 antibodyBRCA1-interacting protein 1 antibodyBRCA1-interacting protein C-terminal helicase 1 antibodyBRCA1/BRCA2 associated helicase 1 antibodyBRIP 1 antibodyBRIP1 antibodyFANCJ antibodyFANCJ_HUMAN antibodyFanconi anemia group J protein antibodyFLJ90232 antibodyMGC126521 antibodyMGC126523 antibodyOF antibodyProtein FACJ antibody

  see all

Anti-BACH1/BRIP1 antibody images

• 

  Western blot - BACH1 antibody (ab3502)
  All lanes : Anti-BACH1/BRIP1 antibody (ab3502) at 1/1000 dilution
  
  Lane 1 : 293 whole cell lysate
  Lane 2 : 293 whole cell lysate with peptide immunogen
  
  Lysates/proteins at 35 µg per lane.
  
  Secondary
  IRDye 800 conjugated Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 140 kDa
  Observed band size : 105 kDa (why is the actual band size different from the predicted?)