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Western blot - BACH1 antibody (ab3502)
(enlarge)
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Product Name
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BACH1/BRIP1 antibody
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See all BACH1/BRIP1 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to BACH1/BRIP1
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Immunogen
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Synthetic peptide: SKDFTNNDMNQGT, corresponding to amino acids 92-104 of Human BACH 1.
BLAST 'SKDFTNNDMNQGT' with  or 
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Reacts with
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Hu
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500 - 1/3000. Detects a band of approximately 105 kDa (predicted molecular weight: 140 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> BRCT Domain Proteins
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Fanconi's Anemia
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Relevance
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BACH1 is a member of the DEAH helicase family that interacts with BRCA1, in vivo. The BACH1/BRCA1 complex formation contributes to a key function of BRCA1. Therefore, it is likely that BACH1 is a target of germline cancer-inducing mutations.
This Bach1/Brip1 protein should not be confused with the separate Bach1 protein listed under SwissProt O14867. This other protein is a BTB and CNC homolog 1 transcription factor.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Expiration date is one (1) year from date of opening.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BACH1/BRIP1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for BACH1/BRIP1 antibody images, references, products related to ab3502 and other tools.
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BACH1/BRIP1 antibody images:
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 Western blot - BACH1 antibody (ab3502)
All lanes : BACH1/BRIP1 antibody (ab3502) at 1/1000 dilution
Lane 1 : 293 whole cell lysate
Lane 2 : 293 whole cell lysate with peptide immunogen
Lysates/proteins at 35 µg per lane.
Secondary
IRDye 800 conjugated Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 140 kDa
Observed band size : 105 kDa (why is the actual band size different from the predicted?)
Detects a band at 105kD, that is blocked by the immunizing peptide.
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Search PubMed (MEDLINE) for references to BACH1/BRIP1
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BACH1/BRIP1 antibody - more information
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Customer reviews (feedback) regarding BACH1/BRIP1 antibody |
Customer FAQs regarding BACH1/BRIP1 antibody |
Protocols for BACH1/BRIP1 antibody |
Price and availability of products related to BACH1/BRIP1 antibody |
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BACH1/BRIP1 antibody - related products:
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BACH1/BRIP1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about BACH1/BRIP1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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