Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Fanconi's Anemia
Overview
Product name
Anti-BACH1/BRIP1 antibody
See all BACH1/BRIP1 products (4) ...
Description
Rabbit polyclonal to BACH1/BRIP1
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: SKDFTNNDMNQGT, corresponding to amino acids 92-104 of Human BACH 1.
SKDFTNNDMN QGT
General notes
Expiration date is one (1) year from date of opening.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - BACH1 antibody (ab3502)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/3000.Detects a band of approximately 105 kDa (predicted molecular weight: 140 kDa).
Target
Relevance
BACH1 is a member of the DEAH helicase family that interacts with BRCA1, in vivo. BACH1 is a DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. BACH1 acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination, and is involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1. The BACH1/BRCA1 complex formation contributes to a key function of BRCA1. Therefore, it is likely that BACH1 is a target of germline cancer-inducing mutations. This Bach1/Brip1 protein should not be confused with the separate Bach1 protein listed under SwissProt O14867. This other protein is a BTB and CNC homolog 1 transcription factor.
Cellular localization
Nuclear
Alternative names
- ATP dependent RNA helicase BRIP1 antibody
- BACH 1 antibody
- BACH 1 antibody
see all
Database links
- Entrez Gene: 83990 Human
- Omim: 605882 Human
- SwissProt: Q9BX63 Human
- Unigene: 532799 Human
Anti-BACH1/BRIP1 antibody images:
Western blot - BACH1 antibody (ab3502)
All lanes : Anti-BACH1/BRIP1 antibody (ab3502) at 1/1000 dilution
Lane 1 : 293 whole cell lysate
Lane 2 : 293 whole cell lysate with peptide immunogen
Lysates/proteins at 35 µg per lane.
Secondary
IRDye 800 conjugated Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 140 kDa
Observed band size : 105 kDa (why is the actual band size different from the predicted?)
Detects a band at 105kD, that is blocked by the immunizing peptide.
References for Anti-BACH1/BRIP1 antibody (ab3502)
ab3502 has not yet been referenced specifically in any publications.
Publishing research using ab3502? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"