Anti-BACH1/BRIP1 antibody (ab7288)
Overview
- Product nameAnti-BACH1/BRIP1 antibodySee all BACH1/BRIP1 primary antibodies ...
- DescriptionRabbit polyclonal to BACH1/BRIP1
- Tested applicationsWB more details
- Species reactivityReacts with: Human
- Immunogen
Synthetic peptide, corresponding to amino acids 1233-1249 of Human BACH1.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab7288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 140 kDa). |
Target
- FunctionDNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1.
- Tissue specificityUbiquitously expressed, with highest levels in testis.
- Involvement in diseaseDefects in BRIP1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
Defects in BRIP1 are the cause of Fanconi anemia complementation group J (FANCJ) [MIM:609054]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. - Sequence similaritiesBelongs to the DEAD box helicase family. DEAH subfamily.
Contains 1 helicase ATP-binding domain. - Domain4Fe-4S iron-sulfur-binding is required for helicase activity (PubMed:20639400).
- Post-translational
modificationsPhosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated. - Cellular localizationNucleus.
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Database links
- Entrez Gene: 83990 Human
- Omim: 605882 Human
- SwissProt: Q9BX63 Human
- Unigene: 128903 Human
Target information above from: UniProt accession
Q9BX63
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ATP dependent RNA helicase BRIP1 antibodyATP-dependent RNA helicase BRIP1 antibodyBACH 1 antibody
- BRAC 1 Associated C Terminal Helicase 1 antibodyBRAC 1 Associated C Terminal Helicase 1 antibodyBRCA 1 Interacting Protein 1 antibodyBRCA 1 Interacting Protein 1 antibodyBRCA1 binding helicase like protein BACH1 antibodyBRCA1 interacting protein C terminal helicase 1 antibodyBRCA1-associated C-terminal helicase 1 antibodyBRCA1-interacting protein 1 antibodyBRCA1-interacting protein C-terminal helicase 1 antibodyBRCA1/BRCA2 associated helicase 1 antibodyBRIP 1 antibodyBRIP 1 antibodyBRIP1 antibodyFANCJ antibodyFANCJ_HUMAN antibodyFanconi anemia group J protein antibodyFLJ90232 antibodyMGC126521 antibodyMGC126523 antibodyOF antibodyProtein FACJ antibody
see all
Anti-BACH1/BRIP1 antibody images
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Western blot - BACH1 antibody (ab7288)All lanes : Anti-BACH1/BRIP1 antibody (ab7288) at 1/1000 dilution
Lane 1 : recombinant FLAG-tagged human BACH1
Lane 2 : IMR90 cell lysate
Lane 3 : 293 cell lysate
Lane 4 : MC7 cell lysate
Predicted band size : 140 kDa
Exposure time : 5 secondsThis was submitted as part of a review by Dr Ron Drapkin
References for Anti-BACH1/BRIP1 antibody (ab7288)
This product has been referenced in:
- Monteiro LJ et al. The Forkhead Box M1 protein regulates BRIP1 expression and DNA damage repair in epirubicin treatment. Oncogene : (2012). Read more (PubMed: 23108394) »
- van Wietmarschen N et al. The Mammalian Proteins MMS19, MIP18, and ANT2 Are Involved in Cytoplasmic Iron-Sulfur Cluster Protein Assembly. J Biol Chem 287:43351-8 (2012). WB . Read more (PubMed: 23150669) »
