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Anti-BACH2 antibody (ab83364)

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Overview

Product name

Anti-BACH2 antibody
See all BACH2 products (2) ...

Description

Rabbit polyclonal to BACH2

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Cow

Immunogen

Synthetic peptide corresponding to a region within internal sequence amino acids 792-841 (SGRRLEGTDP GTFSERGPPL EPRSQTVTVD FCQEMTDKCT TDEQPRKDYT) of Human BACH2 (NP_068585).

SGRRLEGTDP GTFSERGPPL EPRSQTVTVD FCQEMTDKCT TDEQPRKDYT

Positive control

293T cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - BACH2 antibody (ab83364)Western blot - BACH2 antibody (ab83364) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab83364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/1562500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Transcriptional regulator that acts as repressor or activator. Binds to Maf recognition elements (MARE). Play important roles in coordinating transcription activation and repression by MAFK.

Tissue specificity

B-cell specific.

Sequence similarities

Belongs to the bZIP family. CNC subfamily.
Contains 1 BTB (POZ) domain.
Contains 1 bZIP domain.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ9BYV9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Bach 2 antibody
  • Bach2 antibody
  • BACH2_HUMAN antibody
  • Basic leucine zipper transcription factor 2 antibody
  • BTB and CNC homolog 2 antibody
  • BTB and CNC homology 1 basic leucine zipper transcription factor 2 antibody
  • OTTHUMP00000016867 antibody
  • OTTHUMP00000016868 antibody
  • OTTHUMP00000040591 antibody
  • Transcription regulator protein BACH2 antibody
see all

Anti-BACH2 antibody images:

  Western blot - BACH2 antibody (ab83364)

Western blot - BACH2 antibody (ab83364)

Anti-BACH2 antibody (ab83364) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 93 kDa
Observed band size : >90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa,80 kDa. We are unsure as to the identity of these extra bands.

Gel concentration: 12%

References for Anti-BACH2 antibody (ab83364)

ab83364 has not yet been referenced specifically in any publications.

Publishing research using ab83364? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"