Products:Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands
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ab16082 has been referenced in 5 publications.
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Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels staining positive for BAFF using ab16082.
Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.
Ab16082 detects membrane-bound human BAFF by FACS.
Method: HEK 293T cells (5 x 10e5) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of full length hBAFF (thick line). Cells were incubated on ice for 30min in 50μl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1
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