![Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - BAFF antibody [Buffy 2] (ab16082) image](#ab16082_1.jpg)
![Flow Cytometry - BAFF antibody [Buffy 2] (FITC) (ab16082) image](#ab16082_2.jpg)
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Products:Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands
Overview
Product name
Anti-BAFF antibody [Buffy 2] (FITC)
See all BAFF products (12) ...
Description
Rat monoclonal [Buffy 2] to BAFF (FITC)
Conjugation
FITC
Tested applications
IHC-P, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant soluble protein, corresponding to amino acids 83 - 285 of Human BAFF.
Positive control
Ramos cells, HL60 AML cells
General notes
1 test means: 1µl of a 1mg/ml stock solution were used to stain 200,000 cells in a sample volume of 50µl.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Clonality
Monoclonal
Clone number
Buffy 2
Isotype
IgM
Research areas
Cancer >> Growth factors >> TNF
Immunology >> Innate Immunity >> Cytokines >> Necrosis Factors
Immunology >> Adaptive Immunity >> B Cells >> Non-CD
Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IHC-P: 1/50. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use streptavidin-biotin amplification (see recommended protocol).
Flow Cyt: Use at a concentration of 1 µg/ml.
Incubate cells on ice for 30 min in 50 µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1µg/ml of Buffy-2-FITC antibody (testing conditions for HEK 293T cells (5 x 105) mock transfected or transfected with an expression plasmid enabling surface expression of full length hBAFF).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
Tissue specificity
Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.
Sequence similarities
Belongs to the tumor necrosis factor family.
Post-translational
modifications
The soluble form derives from the membrane form by proteolytic processing.
N-glycosylated.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionQ9Y275
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- soluble form antibody
- ApoL related ligand TALL 1 antibody
- B cell Activating Factor antibody
see all
Database links
- Entrez Gene: 10673 Human
- Entrez Gene: 24099 Mouse
- Omim: 603969 Human
- SwissProt: Q9Y275 Human
- SwissProt: Q9WU72 Mouse
- Unigene: 525157 Human
- Unigene: 596110 Human
- Unigene: 28835 Mouse
Anti-BAFF antibody [Buffy 2] (FITC) images:
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - BAFF antibody [Buffy 2] (ab16082)
![Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - BAFF antibody [Buffy 2] (ab16082)](/ps/datasheet/Images/16/ab16082/ab16082_1.jpg)
Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels staining positive for BAFF using ab16082.
Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.
Flow Cytometry - BAFF antibody [Buffy 2] (FITC) (ab16082)
![Flow Cytometry - BAFF antibody [Buffy 2] (FITC) (ab16082)](/ps/datasheet/Images/16/ab16082/ab16082_2.jpg)
Ab16082 detects membrane-bound human BAFF by FACS.
Method: HEK 293T cells (5 x 10e5) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of full length hBAFF (thick line). Cells were incubated on ice for 30min in 50μl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1
References for Anti-BAFF antibody [Buffy 2] (FITC) (ab16082)
This product has been referenced in:
- Huard Bet al. BAFF production by antigen-presenting cells provides T cell co-stimulation. Int Immunol 16:467-75 (2004).Read more (PubMed: 14978020) »
- Novak AJet al. Expression of BLyS and its receptors in B-cell non-Hodgkin lymphoma: correlation with disease activity and patient outcome. Blood 104:2247-53 (2004).Read more (PubMed: 15251985) »
See all 5 publications for this product
Publishing research using ab16082? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"