Anti-BAFF antibody [Buffy 2] (ab16081)

Product code: 119904
Inquiry: May i please have more information on how this antibody was tested on IHC-P. What was the specific type of antigen retrieval that was used? Could you share the IHC-P protocol that was used?

ANSWER:

Thank you for contacting us.

I have some further information to share with you in regards to the antibodies ab119904, ab79056 and ab86574.

Anti-CD134 / OX40 (ab119904)

This antibody was used in staining of paraffin embedded formalin fixed tissues using sodium citrate buffer (10 mM, pH6) for 15 minutes for antigen retrieval.

Anti-IL17 (ab79056)

This antibody has successfully been used to stain dog lymph node tissue of formalin fixed, paraffin embedded tissue sections again using citrate buffer as the antigen retrieval agent.

Anti-TNFRSF18 (ab86574)

I am seeking further information on what antigen retrieval was used with this antibody and will let you know as soon as I have further details.

As we have discussed previously, it may be that these conditions are not optimal with the tissues you are hoping to use and some optimisation of the protocol will most likely need to be performedin order to achieve the best results.

I have noticed that the antibodies you have chosen have quite different species listed as compatible with the antibody. If you would be able to share with me exactly what samples you intend to use with your staining I can have a look to see if these are the most appropriate antibodies to use or if we have alternatives which may be preferable.

I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

ab16081 gives no staining in IHC-Fr

ANSWER:

Thank you for contacting us.

Your credit note ID is 20547.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Thanks again for your kind help.
I would like your opinion regarding what antibody you would be thebest replacement to ab8396 for Human WB.
As far as I see http://www.abcam.com/BAFF-antibody-Buffy-2-ab16081.html, http://www.abcam.com/BAFF-antibody-Buffy-XI-ab16087.html,http://www.abcam.com/BAFF-antibody-ab65360.htmlhave the best WB figures, do you have any additional data that might help the customer choose?

Warm regards,

ANSWER:

Thank you for your reply.

Regarding the three options mentioned, product ab65360 is not currently in stock, and it is yet to be confirmed the estimated time of arrival.

Product ab16081 is a good seller with low rate of complaints, but unfortunately there isn’t any WB mage available on its datasheet. Ab16087 has been specifically validated for WB, and contains an image on its datasheet, which can be helpful to users.

In any case, any of the options chosen should work in WB with Human species, as it is specified on the datasheets. In case they didn’t, our Abpromise guarantee would ensure a replacement or credit note in compensation if the complaint is reported within 6 months of purchase.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Is this rat IgM antibody a monomer or pentamer?

ANSWER:

Thank you for your patience with this reply.

I finally heard back from the lab as they were looking into this further. The production manager said that IgMs really are pentameric and nothing else, but it is possible that there could potentially be things other than IgMs present in the mixture. There is some difficulty in purifying IgMs, so these antibodies are prepared by precipitating all the proteins from the ascites, and might contain heavy chain portions of the antibody. So while the answer is “The antibody in question is only present as a pentamer,” there might be things that look like (at least parts of) monomers present. So if you run this product in PAGE, you will see a major band corresponding to IgM pentamers, and possibly some additional Fc portions and other proteins that have slipped through the purification process.

I hope this information helps. Please contact us with any other questions.  

How has this antibody has been purified? Was it by ammonium sulfate precipitation?

ANSWER:

This antibody was purified by ammonium sulfate precipitation. New lots of the antibody are tested by WB.