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Products:Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands
Overview
Product name
Anti-BAFF antibody
See all BAFF products (12) ...
Description
Rabbit polyclonal to BAFF
Tested applications
Sandwich ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Sheep, Rabbit, Cow, Pig, Chimpanzee, Rhesus monkey
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human BAFF.
(Peptide available as ab69714.)
Positive control
This antibody gave a positive signal in the following Human Whole Cell Lysates: Jurkat, HL60, MOLT4
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Growth factors >> TNF
Immunology >> Innate Immunity >> Cytokines >> Necrosis Factors
Immunology >> Adaptive Immunity >> B Cells >> Non-CD
Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA: Use a concentration of 0.1 µg/ml Can be paired for Sandwich ELISA with Mouse monoclonal to BAFF (ab9359). (For sandwich ELISA, use this antibody as Detection at 0.1µg/ml with ab9359 as Capture.)
WB: Use a concentration of 1 µg/mlDetects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
Target
Function
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
Tissue specificity
Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.
Sequence similarities
Belongs to the tumor necrosis factor family.
Post-translational
modifications
The soluble form derives from the membrane form by proteolytic processing.
N-glycosylated.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionQ9Y275
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- soluble form antibody
- ApoL related ligand TALL 1 antibody
- B cell Activating Factor antibody
see all
Database links
- Entrez Gene: 100101577 Cow
- Entrez Gene: 10673 Human
- Entrez Gene: 24099 Mouse
- Entrez Gene: 100038026 Pig
- Entrez Gene: 100101577 Rabbit
- Entrez Gene: 498666 Rat
- Entrez Gene: 780517 Sheep
- Omim: 603969 Human
see all
Anti-BAFF antibody images:
Sandwich ELISA - BAFF antibody (ab65360)
Standard Curve for BAFF (Analyte: ab79745) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal to BAFF (ab9359) at 1ug/ml and Detector Antibody Rabbit polyclonal to BAFF (ab65360) at 0.1ug/ml
Western blot - BAFF antibody (ab65360)
All lanes : Anti-BAFF antibody (ab65360) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 3 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 31 kDa
Observed band size : 31 kDa
Exposure time : 1 minute
Protocols
References for Anti-BAFF antibody (ab65360)
ab65360 has not yet been referenced specifically in any publications.
Publishing research using ab65360? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"