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Western blot - BAFF antibody (ab65360)

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Product Name
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BAFF antibody
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See all BAFF antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to BAFF
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human BAFF. (Peptide available as ab69714.)
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Reacts with
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Hu
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Predicted to react
(species key)
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Rat, Chmp, Rb, RMk (Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following Human Whole Cell Lysates: Jurkat, HL60, MOLT4
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Cellular localization
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Cell Membrane; single pass type II membrane protein and Secreted
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Research areas
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Cancer >> Growth factors >> TNF Immunology >> Innate Immunity >> Cytokines >> Necrosis Factors Immunology >> Adaptive Immunity >> B Cells >> Non-CD Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands
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Relevance
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Members of the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL1 (for TNF- and ApoL- related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NFkB and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell stimulator since it induced B cell proliferation and immunoglobulin secretion. Two receptors for BAFF were recently identified and designated TACI and BCMA. BAFF also signals through a third TNF receptor BAFFR/BR3. BAFF and its receptors are involved in the development of systemic lupus erythaematosus and other B cell associated autoimmune diseases. Like TNFa and TRAIL, THANK was shown to activate NF-kB and c-jun N terminal kinase (JNK) and to induce apoptosis.
The human BAFF gene codes for a 285 amino acid type II transmembrane protein containing a 46 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 218 amino acid extracellular domain.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BAFF antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for BAFF antibody images, references, products related to ab65360 and other tools.
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BAFF antibody images:
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Western blot - BAFF antibody (ab65360)
All lanes : BAFF antibody (ab65360) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate Lane 3 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 31 kDa Observed band size : 31 kDa
Exposure time : 1 minute
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Search PubMed (MEDLINE) for references to BAFF
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BAFF antibody - more information
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Customer reviews (feedback) regarding BAFF antibody |
Customer FAQs regarding BAFF antibody |
Protocols for BAFF antibody |
Price and availability of products related to BAFF antibody |
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BAFF antibody - related products:
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BAFF antibody - other tools:
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Contact Abcam with a Technical Enquiry about BAFF antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"