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Overview

  • Product nameAnti-BAFF antibodySee all BAFF primary antibodies ...
  • Description
    Rabbit polyclonal to BAFF
  • Tested applicationsSandwich ELISA, WB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Cow, Pig, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human BAFF.

    (Peptide available as ab69714.)

  • Positive controlThis antibody gave a positive signal in the following Human Whole Cell Lysates: Jurkat, HL60, MOLT4

Properties

Applications

Our Abpromise guarantee covers the use of ab65360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA sELISA: Use a concentration of 0.1 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal to BAFF (ab9359). For sandwich ELISA, use this antibody as Detection at 0.1µg/ml with ab9359 as Capture.
WB WB: Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).Can be blocked with BAFF peptide (ab69714).

Target

  • Alternative names
      ApoL related ligand TALL 1 antibodyB cell Activating Factor antibodyB lymphocyte stimulator antibody
      B-cell-activating factor antibodyBAFF antibodyBLYS antibodyCD 257 antibodyCD257 antibodyCD257 antigen antibodydelta BAFF antibodyDendritic cell derived TNF like molecule antibodyDendritic cell-derived TNF-like molecule antibodyDTL antibodyDTL precursor antibodyPRO738 antibodysoluble form antibodyTALL 1 antibodyTALL-1 antibodyTALL1 antibodyTHANK antibodyTN13B_HUMAN antibodyTNF and APOL related leukocyte expressed ligand 1 antibodyTNF homolog that activates apoptosis antibodyTNF homolog that activates apoptosis NKFB and JNK. antibodyTNF- and APOL-related leukocyte expressed ligand 1 antibodyTNFSF13B antibodyTNFSF20 antibodyTumor necrosis factor ligand superfamily member 13B antibodyTumor necrosis factor ligand superfamily member 20 antibodyTumor necrosis factor like protein ZTNF4 antibodyTumor necrosis factor superfamily member 13B antibodyUNQ401 antibodyZTNF4 antibody
    see all

Anti-BAFF antibody images

  • Standard Curve for BAFF (Analyte: ab79745) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal to BAFF (ab9359) at 1ug/ml and Detector Antibody Rabbit polyclonal to BAFF (ab65360) at 0.1ug/ml
  • All lanes : Anti-BAFF antibody (ab65360) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
    Lane 3 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 31 kDa


    Exposure time : 90 seconds

Protocols

References for Anti-BAFF antibody (ab65360)

ab65360 has not yet been referenced specifically in any publications.

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