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Products:Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands
Overview
Product name
Anti-BAFF antibody
See all BAFF products (12) ...
Description
Mouse monoclonal to BAFF
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
TNFSF13B (AAH20674, 1 a.a. ~ 286 a.a) full length recombinant protein with tag. MW of the tag is 26kDa.
Positive control
BAFF transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Growth factors >> TNF
Immunology >> Innate Immunity >> Cytokines >> Necrosis Factors
Immunology >> Adaptive Immunity >> B Cells >> Non-CD
Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands
Applications
Show applications key
Our Abpromise guarantee covers the use of ab68345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).
ELISA: Use at an assay dependent dilution.
Target
Function
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
Tissue specificity
Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.
Sequence similarities
Belongs to the tumor necrosis factor family.
Post-translational
modifications
The soluble form derives from the membrane form by proteolytic processing.
N-glycosylated.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionQ9Y275
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- soluble form antibody
- ApoL related ligand TALL 1 antibody
- B cell Activating Factor antibody
see all
Database links
- Entrez Gene: 10673 Human
- Omim: 603969 Human
- SwissProt: Q9Y275 Human
- Unigene: 525157 Human
- Unigene: 596110 Human
Anti-BAFF antibody images:
Western blot - BAFF antibody (ab68345)
Anti-BAFF antibody (ab68345) at 1/500 dilution + immunogen of 57kDa at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 31 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Western blot - BAFF antibody (ab68345)
All lanes : Anti-BAFF antibody (ab68345) at 1/500 dilution
Lane 1 : BAFF transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 31 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
References for Anti-BAFF antibody (ab68345)
ab68345 has not yet been referenced specifically in any publications.
Publishing research using ab68345? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"