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Western blot - BAFF antibody (ab8396)
Western blot - BAFF antibody (ab8396)
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Immunocytochemistry/ Immunofluorescence - BAFF antibody (ab8396)
Immunocytochemistry/ Immunofluorescence - BAFF antibody (ab8396)
(enlarge)


Product Name 

BAFF antibody

 

See all BAFF antibodies (10)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to BAFF

Immunogen 

Synthetic peptide, corresponding to amino acids 254-269 of Human BAFF.

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

ICC/IF, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 34 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HL60 cell lysate

Cellular localization 

Cell Membrane; single pass type II membrane protein and Secreted

Research areas 

Cancer >> Growth factors >> TNF
Immunology >> Innate Immunity >> Cytokines >> Necrosis Factors
Immunology >> Adaptive Immunity >> B Cells >> Non-CD
Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands

Relevance 

Members of the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL1 (for TNF- and ApoL- related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NFkB and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell stimulator since it induced B cell proliferation and immunoglobulin secretion. Two receptors for BAFF were recently identified and designated TACI and BCMA. BAFF also signals through a third TNF receptor BAFFR/BR3. BAFF and its receptors are involved in the development of systemic lupus erythaematosus and other B cell associated autoimmune diseases. Like TNFa and TRAIL, THANK was shown to activate NF-kB and c-jun N terminal kinase (JNK) and to induce apoptosis.

The human BAFF gene codes for a 285 amino acid type II transmembrane protein containing a 46 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 218 amino acid extracellular domain.

 

Alternative names BAFF antibody (ab8396)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

10673    (Human)
24099    (Mouse)
498666    (Rat)

Omim

    

603969    (Human)

SwissProt

    

Q9Y275    (Human)
Q9WU72    (Mouse)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

PBS with 0.02% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

Notes 

BAFF is also known as B cell Activating Factor

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BAFF antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for BAFF antibody images, references, products related to ab8396 and other tools.

 

BAFF antibody images:

Product image  Western blot - BAFF antibody (ab8396)

Western blot - BAFF antibody (ab8396)

All lanes : BAFF antibody (ab8396) at 1 µg/ml

Lane 1 : Human HL60 cell lysate
Lane 2 : Murine spleen tissue lysate


Observed band size : 34 kDa (why is the actual band size different from the predicted?)

 

Product image  Immunocytochemistry/ Immunofluorescence - BAFF antibody (ab8396)

Immunocytochemistry/ Immunofluorescence - BAFF antibody (ab8396)

ab8396 at 1µg/ml staining BAFF in HL60 cells by ICC/IF

 
 

Search PubMed (MEDLINE) for references to BAFF

 

BAFF antibody - more information

Customer reviews (feedback) regarding BAFF antibody

Customer FAQs regarding BAFF antibody

Protocols for BAFF antibody

Price and availability of products related to BAFF antibody

 

BAFF antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Immunizing Peptide for BAFF antibody

ab8397

  

BAFF (C-term) peptide (human)

 

Positive Controls for BAFF antibody

ab7914

  

HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate (Human) Whole Cell Lysate

 

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ab6564

  

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ab6717

  

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Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

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Chromeo™ 642 antibody

 

Other BAFF Antibodies

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BAFF antibody - other tools:

Contact Abcam with a Technical Enquiry about BAFF antibody

 

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