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Products:Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands
Overview
Product name
Anti-BAFF antibody
See all BAFF products (12) ...
Description
Rabbit polyclonal to BAFF
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide, corresponding to amino acids 254-269 of Human BAFF.
Positive control
HL60 cell lysate
General notes
BAFF is also known as B cell Activating Factor
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Light chain type
unknown
Research areas
Cancer >> Growth factors >> TNF
Immunology >> Innate Immunity >> Cytokines >> Necrosis Factors
Immunology >> Adaptive Immunity >> B Cells >> Non-CD
Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: Use a concentration of 0.25 - 1 µg/ml.Detects a band of approximately 34 kDa.
Target
Function
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
Tissue specificity
Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.
Sequence similarities
Belongs to the tumor necrosis factor family.
Post-translational
modifications
The soluble form derives from the membrane form by proteolytic processing.
N-glycosylated.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionQ9Y275
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- soluble form antibody
- ApoL related ligand TALL 1 antibody
- B cell Activating Factor antibody
see all
Database links
- Entrez Gene: 10673 Human
- Entrez Gene: 24099 Mouse
- Entrez Gene: 498666 Rat
- Omim: 603969 Human
- SwissProt: Q9Y275 Human
- SwissProt: Q9WU72 Mouse
- Unigene: 525157 Human
- Unigene: 596110 Human
see all
Anti-BAFF antibody images:
Western blot - BAFF antibody (ab8396)
All lanes : Anti-BAFF antibody (ab8396) at 1 µg/ml
Lane 1 : Human HL60 cell lysate
Lane 2 : Murine spleen tissue lysate
Observed band size : 34 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - BAFF antibody (ab8396)
ab8396 at 1µg/ml staining BAFF in HL60 cells by ICC/IF
References for Anti-BAFF antibody (ab8396)
ab8396 has not yet been referenced specifically in any publications.
Publishing research using ab8396? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"