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Overview

  • Product nameAnti-BAFF antibodySee all BAFF primary antibodies ...
  • Description
    Rabbit polyclonal to BAFF
  • Tested applicationsICC/IF, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 254-269 of Human BAFF.

  • Positive controlHL60 cell lysate
  • General notesBAFF is also known as B cell Activating Factor

Properties

Applications

Our Abpromise guarantee covers the use of ab8396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: Use a concentration of 1 µg/ml.
WB WB: Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 34 kDa.Can be blocked with BAFF (C-term) peptide (human) (ab8397).

Target

  • FunctionCytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
  • Tissue specificityAbundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    modifications    The soluble form derives from the membrane form by proteolytic processing.
    N-glycosylated.
  • Cellular localizationSecreted and Cell membrane.

    • Target information above from: UniProt accession Q9Y275 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      ApoL related ligand TALL 1 antibodyB cell Activating Factor antibodyB cell Activating Factor antibody
      B lymphocyte stimulator antibodyB-cell-activating factor antibodyBAFF antibodyBLYS antibodyCD 257 antibodyCD257 antibodyCD257 antigen antibodydelta BAFF antibodyDendritic cell derived TNF like molecule antibodyDendritic cell-derived TNF-like molecule antibodyDTL antibodyDTL precursor antibodyPRO738 antibodysoluble form antibodyTALL 1 antibodyTALL-1 antibodyTALL1 antibodyTHANK antibodyTN13B_HUMAN antibodyTNF and APOL related leukocyte expressed ligand 1 antibodyTNF homolog that activates apoptosis antibodyTNF homolog that activates apoptosis NKFB and JNK. antibodyTNF- and APOL-related leukocyte expressed ligand 1 antibodyTNFSF13B antibodyTNFSF20 antibodyTumor necrosis factor ligand superfamily member 13B antibodyTumor necrosis factor ligand superfamily member 20 antibodyTumor necrosis factor like protein ZTNF4 antibodyTumor necrosis factor superfamily member 13B antibodyUNQ401 antibodyZTNF4 antibody
    see all

Anti-BAFF antibody images

  • Western blot - BAFF antibody (ab8396)
    Western blot - BAFF antibody (ab8396)
    All lanes : Anti-BAFF antibody (ab8396) at 1 µg/ml

    Lane 1 : Human HL60 cell lysate
    Lane 2 : Murine spleen tissue lysate


    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • Immunocytochemistry/ Immunofluorescence - BAFF antibody (ab8396)
    Immunocytochemistry/ Immunofluorescence - BAFF antibody (ab8396)

    ab8396 at 1µg/ml staining BAFF in HL60 cells by ICC/IF

References for Anti-BAFF antibody (ab8396)

ab8396 has not yet been referenced specifically in any publications.

Publishing research using ab8396 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

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Thank you for caring and providing an excellent service (as always).
The customer has tried to implement Abcam's suggestion but still got unspecific results.
We would very much appreciate to go ahead with your offer to get a replacement antibody...

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Thank you for your reply.

I will be happy to send a replacement if the product is still under the guarantee Abpromise (after 6 months of the purchase date). Could you please provide the order number?

Would you prefer receiving a new lo...

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Dear Tech support,   One of our customers has ordered ab8396 and needs a positive control for it for WB. Which of the available Baff protein/peptides would you recommend? The product ab8397 seems to have the exact amino acid sequence used as an immonogen ...

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Thank you for your inquiry. The ab8396 can only be used for blocking. Indeed, the peptide is very short containing only 16 amino acids. I would therefore not recommend it for using as a control by loading on the gel. It would run at around 1.8kD - which i...

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I need to find a Baff antibody which can be used for IHC. Has ab7357 or ab8396 been tested for IHC? Thanks.

Thank you for your enquiry. To our knowledge, neither of these antibodies have yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase one of these products, please let us know h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"