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Products:Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands
Overview
Product name
Anti-BAFF antibody
See all BAFF products (12) ...
Description
Mouse monoclonal to BAFF
Tested applications
ELISA, Sandwich ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human).
Positive control
HL60 cell lysate
Properties
Form
Lyophilised:
Reconstitute with 500ul distilled water.
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Phosphate buffered saline
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Growth factors >> TNF
Immunology >> Innate Immunity >> Cytokines >> Necrosis Factors
Immunology >> Adaptive Immunity >> B Cells >> Non-CD
Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands
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Sandwich ELISA - BAFF antibody (ab9359)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab9359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
sELISA: Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Rabbit polyclonal to BAFF (ab65360). (For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab65360 as Detection.)
Target
Function
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
Tissue specificity
Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.
Sequence similarities
Belongs to the tumor necrosis factor family.
Post-translational
modifications
The soluble form derives from the membrane form by proteolytic processing.
N-glycosylated.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionQ9Y275
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- soluble form antibody
- ApoL related ligand TALL 1 antibody
- B cell Activating Factor antibody
see all
Database links
- Entrez Gene: 10673 Human
- Omim: 603969 Human
- SwissProt: Q9Y275 Human
- Unigene: 525157 Human
- Unigene: 596110 Human
Anti-BAFF antibody images:
Sandwich ELISA - BAFF antibody (ab9359)
Standard Curve for BAFF (Analyte: ab79745) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal to BAFF (ab9359) at 1ug/ml and Detector Antibody Rabbit polyclonal to BAFF (ab65360) at 0.1ug/ml
Protocols
References for Anti-BAFF antibody (ab9359)
ab9359 has not yet been referenced specifically in any publications.
Publishing research using ab9359? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"