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BAFF protein (Active) (ab79745)

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Overview

Product name

BAFF protein (Active)
See all BAFF products (7) ...

Protein description

Recombinant full length Human soluble BAFF protein (aa134-285) 17kDa. (SwissProt ID Q9Y275).

Expression host

E. coli

Properties

Purity

> 95% by SDS-PAGE

Purification notes

Purity is >98% by SDS-PAGE and HPLC analysis.

Form

Lyophilised

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: None
Constituents: 0.5mM DTT, 1X PBS

Concentration

Concentration information loading...

Additional notes

Endotoxin Level: < 1EU per µg of BAFF as determined by the LAL method.

Research areas

Stem Cells >> Stem Cell Cytokines >> Recombinant Human
Cancer >> Growth factors >> TNF
Immunology >> Innate Immunity >> Cytokines >> Necrosis Factors
Immunology >> Adaptive Immunity >> B Cells >> Non-CD
Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands

  • Sandwich ELISA - BAFF protein (Active) (ab79745)Sandwich ELISA - BAFF protein (Active) (ab79745) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab79745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • SDS-PAGE

     

  • Functional Studies

     

Application notes

Biological Activity: Measured by its ability to stimulate human B lymphocyte cells. The ED50 of this effect is between 5 - 10 ng/ml.
SDS-PAGE: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Protein info

Function

Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.

Tissue specificity

Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.

Sequence similarities

Belongs to the tumor necrosis factor family.

Post-translational
modifications

The soluble form derives from the membrane form by proteolytic processing.
N-glycosylated.

Cellular localization

Secreted and Cell membrane.

Target information above from: UniProt accessionQ9Y275 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

BAFF protein (Active) images:

  Sandwich ELISA - BAFF protein (Active) (ab79745)

Sandwich ELISA - BAFF protein (Active) (ab79745)

Standard curve for BAFF (Analyte: ab79745) dilution range 1pg/ml to 1µg/ml using Capture Antibody ab9359 at 1µg/ml and Detector Antibody ab96360 at 0.1µg/ml.

References for BAFF protein (Active) (ab79745)

ab79745 has not yet been referenced specifically in any publications.

Publishing research using ab79745? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"